N-(4-羧基苯)钐维甲酰胺诱导HL-60细胞凋亡及其分子机制研究  

Hl-60 Apoptosis Induced by Samarium Complex of N-(4-carboxyphenyl) Retinami and its Molecular Mechanism

作　　者：李成檀[1] 李文惠[1] 吴志豪[1] 孙雨葳[1] 

机构地区：[1]兰州医学院生物化学与分子生物学教研室,730000

出　　处：《实用癌症杂志》2003年第1期11-12,18,共3页The Practical Journal of Cancer

摘　　要：目的　探讨N -(4 -羧基苯 )钐维甲酰胺对人早幼粒白血病细胞株HL -60细胞的诱导凋亡及其作用机制。方法　通过光镜、流式细胞仪、琼脂糖凝胶电泳、RT -PCR等观察并分析N -(4 -羧基苯 )钐维甲酰胺对HL -60细胞的作用。结果　N-(4 -羧基苯 )钐维甲酰胺能诱导HL -60细胞凋亡 ,并使细胞bcl -2表达下降。结论　N -(4 -羧基苯 )钐维甲酰胺引起的HL-60细胞凋亡的分子机制可能是通过下调bclObjective To investigate the effects of samarium complex of N (4 carboxyphenyl) retinamide on apoptosis of HL 60 cell and its molecular mechanism.Methods HL 60 cells were treated with Sm complex,then observed and analysed by light microscopy,flow cytometry,agarose gel electrophoresis,and RT PCR.Results The cells showed characters of apoptosis as treated by samarium complex of N (4 carboxyphenyl) retinamide,bcl 2 and its mRNA declined with the dose increasing.Conclusion Sm complex has antitumor effect on HL 60,and it is probably carried out via down regulating bcl 2.

关 键 词：N-(4-羧基苯)钐维甲酰胺 HL-60细胞 细胞凋亡 

分 类 号：R730.231[医药卫生—肿瘤]