宫颈鳞癌及癌前病变组织中VEGF、nm23表达及微血管密度研究  

The Expression of VEGF,nm23 and the Study of Microvessel Density in Cervical Squamous Carcinoma and Cervical Precancerous Lesions

作　　者：石新兰[1] 黄利鸣[1] 黎家华[1] 徐弘 邓青[3] 

机构地区：[1]三峡大学医学院病理教研室,443003 [2]宜昌市第二人民医院,443003 [3]三峡大学医学院预防医学教研室,443003

出　　处：《实用癌症杂志》2003年第1期43-46,共4页The Practical Journal of Cancer

基　　金：湖北省卫生厅资助项目 (WJ0 1 589)

摘　　要：目的　探讨宫颈鳞癌及癌前病变组织中VEGF、nm 2 3表达情况及MVD与宫颈鳞癌发展浸润的关系。方法　采用免疫组化方法对 5 0例宫颈鳞癌、2 8例CIN和 12例正常宫颈组织进行VEGF、nm 2 3和CD3 4检测 ,计数癌组织MVD ,并结合临床资料进行统计分析。结果　宫颈鳞癌VEGF阳性表达率明显高于正常组织和CINⅠ、Ⅱ级组织 ,VEGF表达与癌组织MVD、肿瘤浸润、转移和临床分期密切相关 ;癌组织MVD与肿瘤转移密切相关 ;正常宫颈组织nm 2 3均呈阳性表达 ,而宫颈浸润癌组织nm 2 3表达率明显降低 ,但nm 2 3表达与肿瘤浸润、转移和临床分期无明显相关性。结论　宫颈鳞癌组织VEGF呈高表达、nm 2 3呈低表达 ,可能在宫颈鳞癌血管生长。Objective To study the expression of vascular endothelial growth factor(VEGF),nm23 in cervical squamous carcinoma and cervical precancerous lesions and the relationship between microvessel density in cervical squamous carcinoma and the angiogenesis of the tumor.Methods 50 cases of cervical squamous carcinoma,28 cases of cervical intraepithelial neoplasia (CIN) and 12 cases of normal cervical tissue sample were stained for VEGF,nm23 and CD34 by immunohistochemistry method.Quantitation of microvessel was performed and analyzed in a conjunction with the clinical features of the tumor.Results VEGF positive expression rate in cervical squamous carcinoma was significantly higher than that in normal cervical tissue and CINⅠ?Ⅱ tissue.VEGF expression was correlated to MVD,invasion,metastasis and the clinical stage of the tumor.MVD in the tumor was correlated to metastasis of the tumor.The positive expression rate of nm23 in normal cervical tissue was 100.0%,but it was remarkably decreased in cervical squamous carcinoma.Our analysis indicated that no significant relationship was found between the state of nm23 expression and invasion,metastasis and the clinical stage of the tumor.Conclusion The higher expression of VEGF and the lower expression of nm23 in cervical squmous carcinoma may play an important role in the microvessel growth and angiogenesis of cervical squamous carcinoma.

关 键 词：宫颈鳞癌 血管内皮生长因子 NM23蛋白 微血管密度 

分 类 号：R737.33[医药卫生—肿瘤]