用芯片技术分析鼻咽癌周围的基质细胞基因表达特点  

Analysis of Characteristics of Gene Expression in Pericancerous Stromal Cells of Nasopharyngeal Carcinoma by cDNA Array

作　　者：李虹[1] 韩为农[1] 冯湘玲[1] 周文[1] 姚开泰[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所分子病理学研究室

出　　处：《癌症》2003年第3期235-238,共4页Chinese Journal of Cancer

基　　金：国家自然科学基金重点项目(编号39730200)

摘　　要：背景与目的:鼻咽的基质细胞是如何参与鼻咽癌的癌变过程一直是研究的热点,本研究利用芯片技术分析鼻咽癌周围的基质细胞基因表达特点,探讨基质细胞在鼻咽癌发生和发展中的可能机制。方法:采用atlashumanselecttumorarray滤膜,通过比较鼻咽癌组织和鼻咽癌细胞系的基因表达谱,获得鼻咽癌周围的基质细胞基因表达特点。结果:鼻咽癌周围的基质细胞至少可以特异地表达40个基因显示。结论:鼻咽癌周围的基质细胞具有基因的特异表达。BACKGROUND &OBJECTIVE: The mechanism of how stromal cell play an important role in nasopharyngeal carcinogenesis is now a hotspot. This study was designed to elucidate the possible mechanism of stromal cell in the occurrence and progression of nasopharyngeal carcinoma (NPC) through the analysis of the characteristics of gene expression in pericancerous stromal cells of NPC by cDNA array. METHODS: The atlas human select tumor arrays were used to compare the expression profiles between NPC tissue and NPC cell lines. RESULTS: Pericancerous stromal cells in NPC expressed at least 40 genes specifically. CONCLUSION: The specific expression of these genes in pericancerous stromal cells provides energy materials for growth of NPC cells; furthermore, it can accelerate the degradation of extracellular matrix, thus promoting the metastasis of NPC cells.

关 键 词：鼻咽肿瘤 基质细胞 基因表达 

分 类 号：R739.63[医药卫生—肿瘤]