MTT检测超声激活血卟啉对SW-480细胞的杀伤作用  

Effects of hematoporphyrin activated with lower intensity focus ultrasound on SW-480 cells measured by MTT colorimetric assay

作　　者：张坤[1] 齐浩[1] 刘全宏[1] 张军平[2] 李清[1] 李萌[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062 [2]陕西师范大学应用声学研究所,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2003年第1期94-98,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(39870240)

摘　　要：采用1.1、1.6、2.3MHz频率聚焦超声结合血卟啉的方式对体外培养的人结肠癌细胞SW 480进行照射,其介质分别为NaCl溶液、RPMI1640、完全培养基,用MTT法测试了SDT对肿瘤细胞的杀伤效果.结果表明,SW 480细胞在声照强度为1.5W/cm2,频率2.3MHz条件下,介质为NaCl溶液、RPMI1640和完全培养基中都存在超声激活血卟啉增强杀伤肿瘤细胞的效果,且在RPMI1640和完全培养基中这种效果和处理后细胞孵育的时间有一定的相关性.SW480 cells with hematoporphyrin are exposed to focus ultrasound of different frequency (1.1MHz, 1.6 MHz, 2.0 MHz, 2.3 MHz) and the same intensity of 1.5W/cm2. The cell lysis is examined by MTT assay. The experiment results indicate that SW480 cells with hematoporphyrin are remarkably killed by hematopophyrin activated with focus ultrasound of frequency of 2.3 MHz. But this effect is not found in SW480 cells without hematoporphyrin. 

关 键 词：MTT检测 SW-480细胞 杀伤作用 微量培养四唑氮盐检测 聚焦超声 血卟啉 人结肠癌细胞 光动力学疗法 

分 类 号：R735.35[医药卫生—肿瘤] R730.5[医药卫生—临床医学]