向大豆导入深黄被孢霉△^6—脂肪酸脱氢酶基因的初步研究  

A PRELIMINARY STUDY ON TRANSFERRING △6-FATTY ACID DESATURASE GENE FROM MORTIERALLA ISABELLINA INTO SOYBEANS

作　　者：卜云萍[1] 李明春[1] 胡国武[1] 王广科[1] 邢来君[1] 

机构地区：[1]南开大学微生物系,天津300071

出　　处：《大豆科学》2003年第1期11-17,共7页Soybean Science

基　　金：国家自然科学基金项目 ( 3 0 2 0 0 176);天津市自然科学基金重点项目 ( 0 13 80 2 5 11)

摘　　要：通过农杆菌介导法 ,将深黄被孢霉△6-脂肪酸脱氢酶基因导入到栽培大豆吉育 4 3、黑农36、黑农 37等品种。采用多种外植体和感染方法 ,从子叶节和胚轴诱导出丛生芽与再生植株。经过在含 5 0mg L卡那霉素的筛选培养基中连续筛选 ,获得一批转基因植株。经PCR检测和DNA分子杂交分析 ,初步证明外源基因已导入并整合到大豆基因组中。The △ 6-fatty acid desaturase gene fron Mortitieralla isabellina was transferred into cultivars Jiyu 43,Heinong 36,Heinong 37, et al. via Agrobacterium infection by inducting with various explant methods. Adentitious buds and regeneration plant were inducted from cotylendon node, hypocotyl. Kanamycin resistant (Kmr) plantlets were obtained by 3-4 cycles screening in selective condition. PCR and Southern blot analysis from the transferred gene plants proved that foreign gene were transferred and integrated into soybean genome.

关 键 词：大豆 深黄被孢霉 △^6-脂肪酸脱氢酶 农杆菌介导法 转基因植株 

分 类 号：S565.1[农业科学—作物学]