高效液相色谱法测定宝塔菜水苏糖含量  

Content Determination of Stachyose in Stachys sieboldii Miq by HPLC

作　　者：杨宪[1,2] 张雪[3] 余晓东[1] 和七一[1] 杜守颖[2] 陈斌[1] 杨水平[4] 赵瑛[1] 

机构地区：[1]重庆师范大学,重庆401331 [2]北京中医药大学,北京100029 [3]重庆市中药研究院,重庆400065 [4]西南大学,重庆400716

出　　处：《激光杂志》2015年第4期49-51,共3页Laser Journal

基　　金：两江学者"计划专项经费资助;重庆市科学委员会基金项目资助(编号:cstc2014jcyA10007);中国博士后基金项目(编号:2014M560063)

摘　　要：建立HPLC法测定宝塔菜中的水苏糖含量。采用岛津2010AHT色谱仪,菲罗门NH2色谱柱(5.0mm×150mm,4.6μm);流动相乙腈-水(67:33);柱温为30℃,流速为1.0ml/min。以水苏糖进样量X为横坐标,峰面积Y为纵坐标,求得回归方程:Y=10381X+9888.8,水苏糖在5.004μg至400.32μg范围内线性关系良好(R2=0.9993),平均回收率为99.68%,RSD为0.42%。该方法结果准确,可作为宝塔菜中水苏糖含量的测定方法。To establish a HPLC method for the determination of stachyose in stachys sieboldii Miq.The stachyose chromatographic experiments were performed on Phenomenex NH2 column(5.0mm ×150mm,4.6μm),the mobile phase was acetonitrile- aqueous solution(67:33);column temperature was 30℃,the flow rate was 1.0ml/min.The results showed that the linear equations were Y=10381X+9888.8for stachyose.The linear range of stachyose was 5.004~400.32μg(R2=0.9993),the average recovery was 99.68%with RSD of 0.42%.The method is feasible,which can contro1 the quality of stachys sieboldii Miq.

关 键 词：宝塔菜 高效液相色谱法 水苏糖 含量测定 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.72[医药卫生—公共卫生与预防医学]