癌基因bcl-2及抑癌基因p53在宫颈癌组织中的表达及临床意义  

Expression of gene bcl-2 and p53 in tissue of cervical carcinoma

作　　者：李兆艾[1] 李小丽[1] 严宗哲[1] 乔中东[1] 赵敏[1] 

机构地区：[1]山西省妇幼保健院,030013

出　　处：《山西医药杂志》2003年第1期7-8,共2页Shanxi Medical Journal

基　　金：山西省青年自然基金资助项目 (9710 62 )

摘　　要：目的　探讨宫颈癌 bcl- 2和 p5 3基因表达的改变与其临床意义。方法　对 2 4例宫颈癌组织及对照组宫颈组织采用半定量反转录聚合酶链反应 (RT- PCR)法检测 bcl- 2和 p5 3基因蛋白的表达 ,结合临床资料分析基因改变的临床意义。结果　宫颈癌中 bcl- 2基因蛋白表达高于对照组 ,p5 3基因蛋白表达低于对照组 ,其中二者均为 期高于 期 , 期均低于 期和 期 ,P<0 .0 1。结论　 bcl- 2和 p5 3基因的表达在宫颈癌的发生发展中起重要作用 ,在宫颈癌早期 ,bcl- 2主要抑制细胞凋亡 ,而 p5Objective To explore the changes of bcl 2 and p53 expression in the tissue of cervical carcinoma and its significance.Methods Applying semi quantitative technique of reverse transcriptase polymerase chain reaction (RT PCR),expression of bcl 2 and p53 was detected in the tissues of 24 cases cervical carcinoma and those of the normal control group.The results were analyzed combined the clinical materials.Results Gene expression of bcl 2 in carcinoma group was higher than that in the control group,that of p53 was lower than that in the control group.Among the carcinoma group,expression in the Ⅰ stage was higher than that in the Ⅱ stage,the Ⅰ and Ⅱ stages higher than the Ⅲ stage (P<0.01).Conclusion Expression of bcl 2 and p53 plays an important role in occurrence and development of cervical carcinoma.In the early period,bcl 2 inhibits cell apoptosis,but p53 promotes it.

关 键 词：癌基因 抑癌基因 宫颈癌 BCL-2 P53 

分 类 号：R737.33[医药卫生—肿瘤]