草莓采后微粒体膜Ca^(2+)-ATPase活性与膜脂过氧化水平  

Microsomal Membrane Ca^(2+)-ATPase Activity and Lipid Peroxidation Level during Senescence of Postharvest Strawberry Fruit

作　　者：樊秀彩[1] 关军锋[1] 张继澍[2] 李广敏[1] 

机构地区：[1]河北省农林科学院农业物理生理生化研究所,石家庄050051 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《园艺学报》2003年第1期15-18,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 93 3 ) ;河北省农林科学院博士基金资助项目

摘　　要：测定了常温 (2 5℃ )和低温 (4℃ )贮藏的草莓果实中微粒体膜Ca2 + ATP酶 (Ca2 + ATPase)与超氧化物歧化酶 (SOD)活性、超氧阴离子 (O-·2 )产生速率和丙二醛 (MDA)含量的变化。结果显示 ,随果实衰老 ,低温贮藏的果实微粒体膜Ca2 + ATPase总活性和质膜、线粒体膜及液泡膜上的Ca2 + ATPase活性 ,微粒体膜SOD活性和O-·2 产生速率呈先升高后降低的趋势 ,MDA含量变化与之相反 ;常温贮藏的果实Ca2 + ATPase活性和O-·2 产生速率变化较为迅速 ,与低温类似 ,而SOD活性逐步下降 ,MDA含量不断升高。这些表明 ,草莓果实衰老与细胞质内Ca2 +Changes in activities of Ca 2+ ATPase and SOD,O -· 2 production rate and MDA content in microsomal membrane of strawberry fruit stored at normal temperature(25℃)and cold temperature(4℃)were investigated.The results showed that the activities of total microsomal membrane Ca 2+ ATPase,plasmolemma Ca 2+ ATPase,mitochondria Ca 2+ ATPase and tonoplast Ca 2+ ATPase,and of SOD,and O -· 2 production rate all increased at first and then decreased,and MDA content changed in the contrary trend during senescence in fruit stored at 4℃.The changing trends in Ca 2+ ATPase activities and O -· 2 production rate of microsomal membrane in fruit stored at 25℃ were consistent with the fruit stored at 4℃.However,a gradual decrease in SOD activity and increase in MDA content were found during room temperature storage.It is suggested that the fruit senescence was closely correlated with the damage of cytoplasmic Ca 2+ homeostasis and the enhancement of membrane lipid peroxidation in strawberry fruit.

关 键 词：草霉 果实衰老 微粒体膜 CA^2+-ATPASE 膜脂过氧化 酶活性 超氧化物歧化酶 

分 类 号：S668.4[农业科学—果树学] S601[农业科学—园艺学]