肾癌患者外周血中碳酸酐酶9mRNA表达的临床意义  

Clinical significance of MN mRNA expression in peripheral blood of renal cell carcinoma patients

作　　者：向萌[1] 段国兰[1] 陈一戎[2] 王志平[1] 付生军[1] 吴彬 

机构地区：[1]兰州医学院附属第二医院泌尿研究所 [2]甘肃省人民医院泌尿外科 [3]兰州市铁路中心医院外科

出　　处：《中华泌尿外科杂志》2003年第3期191-193,共3页Chinese Journal of Urology

摘　　要：目的　探讨肾细胞癌患者外周血中碳酸酐酶 9(MN)mRNA表达的临床意义。　方法　应用RT PCR技术 ,对 3 0例肾细胞癌患者及 3 0例非肾细胞癌患者外周血中肾癌细胞标志物MNmR NA进行检测。　结果　非肾癌组外周血中均不表达MNmRNA。肾癌患者外周血MNmRNA阳性表达 13例 (43 % ) ,其中肾癌转移 8例 ,局限性肾癌 5例 ;肾透明细胞癌 12例 ,混合型癌 1例。外周血MNmRNA表达与肾癌的临床分期无相关性 (P >0 .0 5 ) ;而肾透明细胞癌阳性率 (12 / 2 2 )明显高于其他组织类型 (P <0 .0 5 )。　结论　RT PCR技术可较敏感地检测到血液中肾癌细胞 ,有助于早期诊断肾癌微小转移。Objective To study the expression of MN mRNA in peripheral blood of renal cell carcinoma(RCC) patients and to evaluate its clinical significance. Methods Peripheral blood MN mRNA expression was detected by means of RT PCR 30 patients with renal cell carcinoma,including 16 with localized RCC and 14 with metastatic RCC,in 30 patients without renal tumors series as controts. Results With RT PCR, MN mRNA expressin was detected in 13 of 30 cases of RCC (43%) including 5 of 16 patients(31%) with localized RCC and 8 of 14 patients(57%) with metastatic RCC, none of the 30 control cases being positive. No correlation was noted between MN results and tumor stage. Conclusions RT PCR assay of MN is a highly sensitive technique for detecting circulating renal carcinoma cells in the peripheral blood, and it may be helpful in the diagnosis of micrometastasis.MN can be regarded as a cell specific relative protein of clear cell renal carcinoma.

关 键 词：肾肿瘤 碳酸酐酶类 聚合酶链反应 

分 类 号：R737.11[医药卫生—肿瘤]