双顺反子人B7-1、IFN-γ共表达载体修饰的卵巢癌瘤苗免疫反应的研究  

The immunological effects of ovarian carcinoma vaccine modified by human B7-1 and IFN-γ gene

作　　者：钱和年[1] 刘广芝[2] 曹善津[1] 冯捷[1] 叶雪[1] 

机构地区：[1]北京大学人民医院妇科肿瘤中心,100044 [2]河南省人民医院妇产科

出　　处：《河南诊断与治疗杂志》2003年第2期79-81,84,共4页Henan Journal of Diagnosis and Therapy

基　　金：国家自然科学基金 (39870 854)资助

摘　　要：目的 :研究B7 1、IFN γ双基因修饰的卵巢癌瘤苗的免疫效果。方法 :构建了双顺反子人B7 1、IFN γ共表达逆转录病毒载体 ,用其修饰卵巢癌原代细胞 ,制备成肿瘤疫苗 ,体外刺激自体淋巴细胞 ,以MTT法进行杀伤抑制试验。对SCID小鼠进行人免疫重建 ,于皮下接种双基因修饰的卵巢上皮癌细胞系 3AO ,观察肿瘤的成瘤情况。结果 :体外试验证明人B7 1、IFN γ基因修饰的卵巢癌疫苗可诱导肿瘤特异性杀伤活性。体内试验观察到双基因修饰的卵巢癌细胞系成瘤性下降。结论 :B7 1、IFN γ双基因修饰的肿瘤细胞可诱导很强抗肿瘤免疫反应 ,为联合免疫基因修饰的卵巢癌瘤苗的制备提供了一定的借鉴。Objective To investigate the antitumor immunological effects of ovarian carcinoma vaccine modified by human B7 1 and IFN γ gene.Methods The bicistronic retroviral vector encoding human B7 1 and IFN γ was constructed.In vitro test:The primary ovarian carcinoma cells were cultured with retrovirus modified to co express B7 1 and IFN γ.Then autologous lymphocytes were irritated with the transduced cells for competition inhibitory cytotoxic test by MTT method.In vivo test:The tumorigenicity of the double gene modified ovarian cell line 3AO/B7 1·IFN was evaluated in human immune reconstituted SCID mice.Results The tumor specific cytotoxic activity was greatly enhanced by double gene modified vaccine.The double gene modification greatly decreased the in vivo tumorigenicity of tumor cells.Conclusions It is suggested that combining B7 1 and IFN γ could be a useful therapeutic approach in ovarian cancer.Further preclinical studies should be done. [

关 键 词：B7-1 IFN-Γ 逆转录病毒载体 卵巢癌瘤苗 免疫治疗 

分 类 号：R737.31[医药卫生—肿瘤]