阿霉素纳米粒逆转P-gp介导的膀胱移行细胞癌多药耐药的实验研究  

Reversion of P-gp mediated MDR of human bladder carcinoma with doxorubicin-loaded polyisobutylcyanoacrylate nanoparticles in vitro

作　　者：张炎[1] 鲁功成[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《临床泌尿外科杂志》2003年第3期167-169,共3页Journal of Clinical Urology

摘　　要：目的 :观察阿霉素聚氰基丙烯酸异丁酯纳米粒 (阿霉素纳米粒 )对多药耐受糖蛋白 (P glycoprotein ,P gp)介导的膀胱移行细胞癌细胞多药耐药 (MDR)的逆转。 方法 :MTT法观察MDR的逆转 ,流式细胞仪检测细胞内阿霉素 (Dox)浓度。结果 :阿霉素纳米对敏感株的细胞毒作用与阿霉素差异无显著性意义 (P >0 .0 5) ;耐药株对阿霉素纳米较对Dox敏感 4 .5倍 (P <0 .0 5) ,细胞内阿霉素浓度显著增高为其机制。结论 :阿霉素纳米可有效逆转P gp介导的MDR。Purpose:To investigate reversion of P gp mediated multidrug resistance of human bladder carcinoma cell lines with Dox PIBCA NP in vitro. Methods:Cytotoxicity of Dox,Dox PIBCA NP and PIBCA NP were determined by measuring the inhibition of cell growth using the MTT assay,and the amount of intracellular Dox accumulation was determined by flow cytometry.Results:In the sensitive cell lines,the toxicity of Dox PIBCA NP run parallel to that of free Dox.In the MDR lines the toxicity of Dox PIBCA NP to cancer cells were 4.5 times more sensitive than that of free Dox,and the increased sensitivity of MDR cells to Dox PIBCA NP was accounted for by a remarkable increase intracellular Dox accumulation.Conclusions:Dox PIBCA NP was effective to reverse P gp mediated MDR of human bladder carcinoma.

关 键 词：阿霉素纳米粒 膀胱移行细胞癌 多药耐药 聚氰基丙烯酸异丁酯纳米粒 多药耐受糖蛋白 

分 类 号：R737.14[医药卫生—肿瘤] R73－361[医药卫生—临床医学]