尼美舒利对胃癌抑制作用的实验研究  

THE INHIBITORY EFFECT OF NIMESULIDE ON GASTRIC CANCER CELLS

作　　者：徐美虹[1] 吴开春[1] 吴汉平[1] 幺立萍[1] 樊代明[1] 

机构地区：[1]第四军医大学西京医院,西安710032

出　　处：《解放军医学杂志》2003年第3期246-248,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39870 32 0 )

摘　　要：为比较尼美舒利与吲哚美辛抑制胃癌的作用及比较尼美舒利对COX 2反义核酸转染前后胃癌细胞生物学行为的影响 ,并探讨其抗癌作用机制 ,用不同浓度尼美舒利和吲哚美辛与SGC790 1共孵育 ;尼美舒利与SGC790 1、790 1 P、790 1 AS共孵育 ,MTT法检测细胞活性。SGC790 1、790 1 P、790 1 AS各自分为尼美舒利组和对照组 ,FACS检测各组细胞周期。电镜观察SGC790 1、790 1 AS及与尼美舒利共孵育后SGC790 1的超微结构。结果显示 ,尼美舒利和吲哚美辛均可抑制胃癌体外增殖 ,效应呈剂量依赖性 ;尼美舒利对 790 1 AS比对亲本细胞的抑制率减少 (P<0 0 5 ) ,使胃癌G1期细胞比例增加(SGC790 1:73 5 %比 5 6 1% ;790 1 AS :76 7%比 66 5 % )。超微结构显示 ,尼美舒利作用的SGC790 1和 790 1 AS分化上发生良性转化 ,且出现凋亡。表明尼美舒利可抑制胃癌细胞体外增殖 ,其作用是COX 2依赖的。抑制胃癌的可能机制之一是抑制COX 2活性 ,进而影响细胞周期使之阻滞于G1期 。The aims of this study were to compare the inhibitory effect of nimesulide and indomethacin on gastric cancer cells, to investigate the effect of nimesulide on SGC7901 and 7901-AS, and to evaluate its probable mechanism. After incubated with nimesulide (0～200μmol/L) or indomethacin (0～25μmol/L), the proliferation of gastric cancer cells was measured by MTT assay. The cell cycle of SGC7901, 7901-P, 7901-AS was respectively observed after being incubated with nimesulide and vehicle control, by using fluorescence activated cell sorter (FACS). Ultramicrostructure of gastric cancer cells (SGC7901,7901-AS,SGC7901+nimesulide) was observed by electronmicroscopy. The results showed that by MTT assay nimesulide, as indomethacin, had dose-dependent inhibitory effects on proliferation of gastric cancer cells. Compared to the inhibitory effect of nimesulide on SGC7901, it was less on 7901-AS (P<0.05). Nimesulide resulted in G 1 delay. The percent of G 1 cells was 73.5% (vs 56.1%) in SGC7901 cells, and 76.7% (vs 66.5%) in 7901-AS cells. Nimesulide also induced differentiation and apoptosis of gastric cancer cells. Therefore, nimesulide as well as indomethacin can dose-dependently inhibit the proliferation of gastric cancer cells. The inhibitory effect is COX-2-dependent. By inhibiting the activation of COX-2 and influencing cell cycle, differentiation and apoptosis of gastric cancer cells are induced, and it might be one of the mechanisms that nimesulide inhibits gastric cancer.

关 键 词：细胞培养 胃肿瘤 尼美舒利 抑制效应肿瘤细胞 实验研究 

分 类 号：R73－36[医药卫生—肿瘤] R735.2[医药卫生—临床医学]