利用微穿刺技术建立猫实验性视网膜脱离及复位模型  

ESTABLISHMENT OF AN EXPERIMENTAL RETINAL DETACHMENT AND REATTACHMENT MODEL USING MICROPIPETTING IN CATS

作　　者：刘铁城[1] 马志中[1] 刘百臣[1] 张晓丽[1] 王常观[1] 胡运韬 范建国 陈华[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《解放军医学杂志》2003年第3期262-263,共2页Medical Journal of Chinese People's Liberation Army

摘　　要：建立猫实验性视网膜脱离及复位动物模型 ,为研究与视网膜脱离和复位相关的视网膜疾病提供合理有效的实验手段。 78只成年猫眼行晶体囊外摘除 ,玻璃体切除手术。 3周后 ,手持尖端直径约 5 0～ 70 μm的玻璃微穿刺针 ,刺入神经视网膜和视网膜色素上皮细胞之间 ,将 0 2 5 %Healon缓慢注入到视网膜下腔 ,造成局部视网膜脱离。 2 4h时后 ,经玻璃体切割机进行眼内气 液交换 ,用尖端直径约 15 0～ 2 0 0 μm的玻璃微穿刺针 ,将视网膜下腔和玻璃体腔内液体吸出 ,填充 3 0 %C3F8使脱离的视网膜重新复位。观察视网膜脱离形态和复位情况以及眼部炎症反应。结果显示 ,视网膜脱离的成功率为 93 6% ( 73 / 78眼 )。脱离 2 4h后 ,视网膜重新复位的成功率为 97 1%( 3 3 / 3 4眼 )。组织学观察发现 ,视网膜脱离 ,位于视网膜光感受器细胞和视网膜色素上皮 (retinalpigmentepithelium ,RPE)细胞之间 ,视网膜重新复位后RPE细胞和光感受器细胞紧密接触。实验表明 ,利用微穿刺技术可以在猫眼上成功建立损伤小、反应轻 。To establish a model of experimental retinal detachment and reattachment, extracapsular lens extraction and vitrectomy were performed in 78 adult cats. After three weeks, a glass micropipette with a flat tip (diameter 150～200μm) was inserted between the neural retina and retinal pigment epithelium (RPE) monolayer. Retinal detachments were produced by subretinal injection of 0.25% solution of Healon through this micropipette. Twenty～four hours later, detached retinas were reattached by air-fluid exchange and subretinal fluid drainage with a glass micropipette. Then 30% perfluoropropane (C 3F 8) gas temponade was porfomed. Retinal detachment was successfully made in 93.6%(73/78), and 97.1%(33/34)of the detached retinas were successfully reattached. By light microscopy, histologic sections showed that seperated retina located between RPE cells and photoreceptors, and neural retina contacted to RPE cells closely in reattached retinas. In conclusion, by using micropuncture, we have established an animal model of retinal detachment and reattachment with minimum injury to the retina, and a high rate of retinal detachment and reattachment were obtained.

关 键 词：疾病模型 动物 视网膜脱离 穿刺术 

分 类 号：R774[医药卫生—眼科]