5-氨基-4-氨甲酰咪唑核苷产生菌AIC-90菌种选育研究  

Screening of An AICAR-Producing Strain AIC-90

作　　者：施庆珊[1] 李良秋[1] 林小平[1] 许虹[1] 邱玉棠[1] 

机构地区：[1]广东省微生物研究所

出　　处：《氨基酸和生物资源》2002年第4期43-46,共4页Amino Acids & Biotic Resources

摘　　要：已肌苷产生菌枯草杆菌GMI- 741(Ade - )为出发菌株 ,通过多因子诱变选育出具有非精确嘌呤缺陷型、丧失腺嘌呤脱氨酶的AICAR(5 -氨基 - 4-氨甲酰咪唑核苷 )产生菌AIC - 90 ,该菌株摇瓶发酵 72h产AICAR可达 2 0 .3g/L以上。An inosine-producing strain GMI-741 was mutagenized for AICAR-producing strain. A non-exacting purine-requiring mutant AIC90, lacking of adenase, produced a large amount of AICAR(5-amino-4-imidazole-carboxamide riboside). On a reciprocal shaker for 72h, the accumulation of AICAR by AIC90 in the culture broth was more than 20.3g/L.

关 键 词：枯草杆菌 AICAR 菌种选育 发酵条件 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]