胰岛素受体底物-1基因3′非翻译区的突变与中国人2型糖尿病的关系  

Relationship between the mutation of insulin receptor substrate-1 gene 3′untranslated region and Type 2 diabetes in the Chinese

作　　者：陈淑华[1] 谷亚鹏[1] 曾卫民[1] 宋惠萍[1] 

机构地区：[1]中南大学湘雅医学院生化教研室,长沙410078

出　　处：《湖南医科大学学报》2003年第1期33-36,共4页Bulletin of Hunan Medical University

基　　金：国家自然科学基金资助项目 (编号 :3 0 0 70 3 5 5 )

摘　　要：目的 :研究胰岛素受体底物 1(IRS 1)基因 3′非翻译区的突变与中国 2型糖尿病的关系。方法 :采用PCR SSCP技术扫描 12 0例中国 2型糖尿病患者和 12 0例正常对照的IRS 1基因 3′非翻译区序列 ,所有SSCP变异的样品进行DNA测序分析。结果 :SSCP分析存在假阳性结果。测序未发现有基因突变。结论 :IRS 1基因 3′非翻译区的突变不是引起中国人Objective To study the relationship between the mutation of the insulin receptor substrate 1(IRS 1) gene 3′untranslated region and Type 2 diabetes in the Chinese. Methods Samples were obtained from 120 Chinese patients with Type 2 diabetes and 120 control subjects. The 3′untranslated region sequence of IRS 1 gene was screened by the polymerase chain reaction (PCR) single strand conformation polymorphism (SSCP) analysis.All SSCP variations were submitted to DNA sequence analysis. Results The SSCP analysis showed false positive results. No gene mutation was found in any of the subjects.Conclusion Mutation in the IRS 1 gene 3′untranslated region is not an important genetic factor for Type 2 diabetes in the Chinese.

关 键 词：基因 突变 胰岛素受体底物-1 3′非翻译区 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]