肿瘤特异性肿瘤/睾丸抗原在肝癌组织中的表达  

Expression of tumor-specific cancer/testis antigens in hepatocellular carcinoma

作　　者：陈红松[1] 覃柳亮[2] 丛旭[1] 王瑜[3] 费然[1] 蒋栋[1] 曹骥[2] 苏建家[2] 魏来[1] 陈慰峰[3] 王宇[1] 

机构地区：[1]北京大学医学部人民医院肝病研究所,北京100044 [2]广西肿瘤防治研究所 [3]北京大学医学部免疫学系

出　　处：《中华肝脏病杂志》2003年第3期145-148,共4页Chinese Journal of Hepatology

基　　金：国家高技术863计划(编号2001AA217151);国家自然基金重点项目(39830420);北京大学医学部"985"项目

摘　　要：目的 研究7种主要肿瘤/睾丸(CT)抗原MAGE-1、MAGE-3、MAGE-4、MAGE-10、NY-ESO-1、SSX-2、SCP-1在原发性肝细胞癌(HCC)患者癌组织中的表达、编码基因的变异状况及与临床指标的关系。 方法 收集30例肝癌患者的癌和癌旁组织,采用特异性引物逆转录聚合酶链反应检测7种CT抗原的表达,并对PCR产物进行测序分析。结果 在30例HCC患者中,MAGE-1、MAGE-3、MAGE-4、MAGE-10、NY-ESO-1、SSX-2、SCP-1在癌组织中的表达率分别为66.7％、70.0％、20.0％、36.7％、40.0％、33.3％和33.3％,而癌旁没有表达。癌组织中至少表达1种、2种和3种CT抗原的阳性率分别为90.0％、70.0％和53.5％。我国肝癌表达的7种CT抗原编码基因与国外报道的相比,同源性非常高。MAGE-10和SCP-1的表达与甲胎蛋白的水平相关,MAGE-3和SSX-2表达与平均年龄相关。 结论 7种CT抗原在HCC患者癌组织中均有表达,阳性率为20.0％-70.0％,其编码基因序列高度保守。一些CT抗原的表达与临床指标存在相关性。Objectives (1) To investigate the expression and gene diversity of the 7 major cancer/testis (CT) antigens, MAGE-1, MAGE-3, MAGE-4, MAGE-10, NY-ESO-1, SSX-2 and SCP-1, in hepatocellular carcinoma (HCC). (2) To analyze the correlations between the clinical characters and CT antigens' expression. Methods The cancer and para-cancer tissues were collected from 30 HCC patients. The mRNAs of seven CT antigens were detected by reverse transcription-polymerase chain reaction (RT-PCR) with the specific primers. The PCR products were sequenced to analyze the CT genes. Results The MAGE-1, MAGE-3, MAGE-4, MAGE-10, NY-ESO-1, SSX-2 and SCP-1 were expressed in 66.7%, 70.0%, 20.0%, 36.7%, 40.0%, 33.3% and 33.3% of the tumor tissues from HCC patients respectively, however, they were not expressed in the para-cancer tissues. Among the 30 patients investigated, 90.0% expressed one CT gene at least, 70.0% expressed two CT genes, and 53.3% expressed three CT genes of the seven CT genes. The coding genes of these CT antigens were highly conserved between in Chinese patients and patients abroad. There were discernible correlations between a -fetoprotein level and MAGE-10 or SCP-1 expression level, as well as between average age and MAGE-3 or SSX-2 expression levels (P < 0.05). Conclusions With a highly conserved coding gene, seven CT antigens were expressed in 20.0%~70.0% of Chinese HCC patients. CT antigens' expression had correlations with some clinical characters.

关 键 词：肝细胞癌 肿瘤特异性抗原 睾丸抗原 编码基因 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]