辐射对abl和bcr基因在IM-9细胞间期核中三维动态分布的影响  

Influence of irradiation on the dynamic three-dimension distribution of abl and bcr genes in the interphase nuclei of IM-9 cell

作　　者：张青[1] 周淑芸[1] 刘晓力[1] 牛超[2] 徐岚[2] 陈赛娟[2] 

机构地区：[1]第一军医大学南方医院血液科,广州510515 [2]上海第二医科大学瑞金医院,上海血液学研究所

出　　处：《中华血液学杂志》2003年第3期144-148,共5页Chinese Journal of Hematology

基　　金：国家自然科学基金资助项目 (3 9770 83 1) ;广东省自然科学基金资助项目 (970 83 3 )

摘　　要：目的　通过观察辐射对bcr和abl基因在间期核内三维分布的影响 ,了解bcr和abl基因发生融合的物质基础 ,探讨慢性髓细胞白血病发病的生物体视学机制。方法　用荧光原位杂交结合激光共聚焦扫描显微镜的方法 ,研究受γ射线照射的IM 9细胞中bcr和abl基因在各细胞周期核内三维空间的分布情况 ,并与正常的IM 9细胞作了比较。结果　abl和bcr基因在间期核内呈非随机分布 ,abl靠近核膜 ,而bcr靠近核心 ,在G0 期两基因最接近 ;同源基因之间的相对距离在增殖期明显大于静止期。照射后abl和bcr基因到核心的相对距离减少 ,两基因间的相对距离亦缩短 ,在S期最接近。结论　辐射会改变bcr和abl基因在间期核内的三维空间分布 ,促使二者互相接近。Objective To investigate the material foundation of the fusion of bcr and abl genes, and to explore the pathogenesis of chronic myeloid leukemia. Methods By FISH combined with laser confocal scanning microscopy, the three dimension(3D) distribution of bcr and abl genes in the interphase nuclei of normal and irradiated IM 9 cells was studied in each cell cycle phases. Results abl and bcr genes distributed non randomly in the interphase nuclei of IM 9 cells. abl gene preferably located at the outer layer and bcr near the core of the nucleus. The two genes were drawn near each other most in G 0 phase. The relative distance between the homologous genes was greater at proliferation phase than at quiescence phase. After irradiation, the relative distances from the two genes to the core and between the two genes were shortened, with the shortest distance between the two genes in S phase. Conclusion Irradiation could change the 3D distribution of abl and bcr genes in the interphase nuclei of IM 9 cell and accelerate them to draw near each other.

关 键 词：辐射 BCR基因 IM-9细胞 三维动态分布 abl基因 荧光原位杂交 激光共聚焦显微镜 细胞间期核内 慢性髓细胞白血病 

分 类 号：R733.72[医药卫生—肿瘤]