IL-12增强HBV融合抗原DNA疫苗在小鼠诱导的细胞免疫应答  

Enhancement of Cellular Immune Responses to Hepatitis B Virus Fusion Antigen DNA Vaccine in Mice by Murine IL-12

作　　者：赵平[1] 高军[1] 王宏卫[1] 曹洁[1] 戚中田[1] 

机构地区：[1]第二军医大学微生物学教研室,上海200433

出　　处：《病毒学报》2003年第1期83-85,共3页Chinese Journal of Virology

基　　金：上海科技发展基金 ( 0 0 43192 0 6 );国家自然科学基金( 39980 0 38)

摘　　要：A plasmid(DNA vaccine)pSC-IL12,coexpressing hepatitis B virus(HBV)envelope-core fusion antigen and murine IL-12,was constructed.After the plasmid was transfected into COS7 cells,HBsAg and HBcAg were detected in cytoplasm,murine IL-12 was detected in supernatant.BALB/c mice were vaccinated intramuscularly with this construct or HBV envelope-core fusion antigen DNA vaccine pSC.Following twice immunizations,humoral and cellular immune responses of the mice were detected.Results showed that pSC-IL12 induced weaker antibody but stronger T lymphocyte proliferative response and cytotoxic T lymphocyte response than pSC did,which indicated that HBV fusion antigen and IL-12 coexpressing DNA vaccine may be more useful for therapy of hepatitis B.A plasmid(DNA vaccine)pSC-IL12,coexpressing hepatitis B virus(HBV)envelope-core fusion antigen and murine IL-12,was constructed.After the plasmid was transfected into COS7 cells,HBsAg and HBcAg were detected in cytoplasm,murine IL-12 was detected in supernatant.BALB/c mice were vaccinated intramuscularly with this construct or HBV envelope-core fusion antigen DNA vaccine pSC.Following twice immunizations,humoral and cellular immune responses of the mice were detected.Results showed that pSC-IL12 induced weaker antibody but stronger T lymphocyte proliferative response and cytotoxic T lymphocyte response than pSC did,which indicated that HBV fusion antigen and IL-12 coexpressing DNA vaccine may be more useful for therapy of hepatitis B.

关 键 词：乙型肝炎病毒 融合抗原 白细胞介素12 DNA疫苗 细胞免疫应答 

分 类 号：R512.620.5[医药卫生—内科学]