淋巴细胞功能相关抗原-1在冻融PMN介导的VEC损伤中的作用  

THE ROLE OF LFA-1 IN THE VASCULAR ENDOTHELIAL CELLS INJURY MEDIATED BY FROZEN/THAWED NEUTROPHILS

作　　者：王敏[1] 刘嘉瀛[1] 杨增仁[1] 颜培华[1] 曹伟[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国应用生理学杂志》2003年第1期52-55,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :探讨组织冻结融化造成血管内皮细胞 (VEC)损伤的机理。方法 :采用密度梯度离心法分离大鼠外周血嗜中性粒细胞 (PMN) ,速率冷冻仪冷冻PMN后水浴复温建立冻融PMN模型。冻融后 4、1 2和 2 4h ,测定PMN表面淋巴细胞功能相关抗原 1 (LFA 1 )的表达 ;将冻融PMN与正常VEC共同孵育后测定培养液中乳酸脱氢酶活性及冻融PMN与VEC的粘附。结果 :①冻融后 2 4h内 ,冻融PMN表面LFA 1的表达呈时间依赖性增强。②冻融PMN与VEC相互作用后 ,PMN VEC粘附明显增强、VEC受到明显损伤。③抗LFA 1单克隆抗体明显抑制冻融PMN与VEC粘附的增强、明显减轻VEC损伤。结论 :冻融可诱发PMN表面LFA 1的表达 ,进而增强PMNAim: To investigate the mechanism of the vascular endothelial cell (VEC) injury caused by freezing/thawing. Methods: The frozen/thawed neutrophil (PMN) model was founded by freezing PMNs with a rate cooling instrument and then rewarming them in a water bath, the PMNs used here were seperated from rat's peripheral blood using density gradients centrifugation techniques. The expression of LFA 1 on the surface of frozen/thawed PMNs was observed at 4 h?12 h and 24 h after freezing/thawing. After coincubating untreated VECs with frozen/thawed PMNs, we detected the VEC injury and the changes in PMN VEC adhesion. Results: ①The PMNs LFA 1 expression increased in a time dependent manner within 24 h after the freezing/thawing of PMNs. ②After coincubating untreated VECs with frozen/thawed PMNs, the adhesion between frozen/thawed PMNs and VECs increased and VEC injury occurred. ③Monoclonal antibody against LFA 1 could block the PMN VEC adhesion and subsequently attenuated the VEC injury. Conclusion: The freezing/thawing of PMNs can elicite an increase in PMN LFA 1 expression and trigger the PMN VEC adhesion and subsequently bring about the VEC injury.

关 键 词：血管内皮细胞 VEC 嗜中性粒细胞 PMN 淋巴细胞功能相关抗原-1 LFA-1 冻融损伤 

分 类 号：R363.2[医药卫生—病理学] R392.1[医药卫生—基础医学]