半肝严重型肝硬化动物模型的建立  

Establishment of animal model with half-liver cirrhosis

作　　者：杨正汉[1] 谢敬霞[2] 周诚[1] 陈敏[1] 章跃武[3] 胡碧芳 莫洪波 吴晓[6] 

机构地区：[1]卫生部北京医院放射科,北京100730 [2]北京大学第三医院放射科 [3]浙江省衢州市人民医院放射科 [4]浙江省义乌市人民医院放射科 [5]广西柳州卫生技术学院 [6]北京大学基础医学院病理学系

出　　处：《中华放射学杂志》2003年第2期170-173,共4页Chinese Journal of Radiology

摘　　要：目的　建立 1种适合于影像学对照研究的肝硬化新模型。方法　 12只犬分成左肝动脉组、右肝动脉组 ,1只犬作为空白对照组。左、右肝动脉组每组各 6只 ,用 4F导管插入左肝动脉或右肝动脉 ,经导管每 2周 1次灌注 5 0～ 10 0 μl四氯化碳 ,定期对动物进行生化、影像学及处死后的病理学观察。结果　随着灌注四氯化碳次数的增加 ,注药的半肝均发生了肝细胞变性坏死、肝脏纤维化及逐渐加重的肝硬化改变 ;而没有灌注四氯化碳的半肝病变程度轻微。该模型用于肝硬化的对照研究既方便又经济。Objective To establish a new cirrhosis model suitable for imaging study. Methods Via a 4 F catheter, 50～100 μl of carbon tetrachloride was injected into the left or right hepatic artery of 12 dogs fortnightly. Liver functional test, imaging study, and pathological examination were performed in these dogs regularly. Results As the times of injection increased, necrosis of hepatocytes, fibrosis, and cirrhosis of the liver aggravated. In each dog, cirrhosis was more serious in the half liver with carbon tetrachloride injection than in the other half liver without carbon tetrachloride injection. With this model, it was convenient to perform the imaging study of liver cirrhosis. Conclusion Animal model with half liver cirrhosis can be established by combining catheter technique and traditional method.

关 键 词：肝硬化 动物实验 四氯化碳 诊断 

分 类 号：R-332[医药卫生]