树、熊猴感染人乙型肝炎病毒血清指征的研究  

Human hepatitis B virus infection of tree shrews and Macaca assamensis in vivo

作　　者：王树声[1] 苏建家 张华远[3] 李媛 张涛[1] 王佑春[3] 黄果勇[1] 覃柳亮 葛宪民[1] 于萍 李河民[3] 

机构地区：[1]广西壮族自治区卫生防疫站 [2]广西肿瘤研究所 [3]中国药品生物制品检定所

出　　处：《中华实验和临床病毒学杂志》2003年第1期21-24,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：国家"九五"科技攻关课题 (96 90 6 30 10 )

摘　　要：目的　验证树、熊猴感染人乙型肝炎 (乙肝 )病毒 (HHBV)血清感染指征。方法　用含HBV的人血清接种给 2 3 3只树 ,2 8只熊猴 ,每周定期采血 ,用不同公司生产的ELISA试剂检测接种后的动物血清感染指标。结果　树感染HHBV后 ,90 .0 %呈急性感染 ,44 .4%为可持续 1年以上的慢性感染 ,3 3 .3 %持续 2年 2个月。熊猴感染HBV后 ,血清HBV标志物呈一过性。结论　熊猴对HHBV并不敏感 ,而树对HHBV高度敏感 ,有望成为HHBV的动物模型。Objective To examine sensitivity of the tree shrews and Macaca assamensis to human hepatitis B virus (HHBV) by serologic methods. Methods Totally 233 tree shrews and 28 Macaca assamensises were inoculated with human sera containing HBV. After inoculation, the sera were collected weekly from them and HBV markers were detected with HBV ditecting ELISA kits. Results Ninety percent of the tree shrews developed acute infection, among them, 44.4% persisted for over one year, 33.3% of them developed chronic infection persisted for 2 years and one month; the persistence of HBV in Macaca assamensises was much shorter. Conclusion These data clearly indicated that tree shrew may be used as an animal model for study of chronic HBV infection,whereas,Macaca assamenises,showed only a transient sensitivity to HHBV.

关 键 词：动物模型 乙型肝炎病毒 树Qu科 猕猴属 

分 类 号：R-332[医药卫生]