9-顺维甲酸对人肺癌组织CyclinD1、Cdk4、P16转录的影响  

Effects of9-cis-retinoic acid on the transcription of cyclinD1,cdk4and p16in cultured human lung cancer tissues and normal tissues

作　　者：贺美兰[1] 刘晓黎 戴洪海[3] 胡国强[3] 张建业[1] 于雪艳[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所 [2]济南金泰生物制药有限公司 [3]山东大学齐鲁医院胸外科

出　　处：《山东大学学报（医学版）》2003年第1期59-61,共3页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(39770730)

摘　　要：目的:检测9-顺维甲酸(9-cis-RA)处理前后体外培养的人肺癌组织及其对照组织细胞周期蛋白因子CyclinD1、细胞周期蛋白依赖性激酶Cdk4、细胞周期蛋白酶抑制因子P16转录水平的变化。探讨其抑制肺癌组织细胞生长的作用机制。方法:采用半定量逆转录聚合酶链式反应(RT-PCR)分析CyclinD1、Cdk4、P16转录的变化。结果:9-cis-RA既可降低体外培养的人肺癌组织Cdk4的转录,又可升高其P16的转录。对肺癌组织和正常肺组织CyclinD1的转录的影响无统计学意义。对正常肺组织Cdk4、P16的转录的影响也无统计学意义。结论:9-cis-RA抑制肺癌组织细胞增殖的作用机理与其调节Cdk4、P16的转录有关。Objective:To detect the changes of the level of cy clinD1,cdk4and P16mRNA ex pres-sion in cul tured human lung cancer thssaes and normat lung tissues before and after treatment with9-cis-RA(1μmol/L),and to learn about the mechanism of9-cis-RA effect against the proliferation of lung can-cer cells.Methods:RT-PCR was used to determine the mRNA expression of cyclinD1,cdk4and P16in human lung tis sue.Results:9-cis-RA can not only reduce the mRNA expression of cdk4but also en hance the mRNA expression of P16in cultured lung cancer tissues,but its effects on mRNA expression of cy-clinD1?cdk4and P16in cultured control tissues and mRNA expression of cyclinD1in cu lured lung can cer tissues have no statistical significance.Conclusion:The inhibito ry effect of9-cis-RA on the growth of lung can cer cells was closely correlated with it is capable of mediating the mRNA tranecription of cdk4and P16.

关 键 词：维甲酸 肺肿瘤 CDK4 P16 细胞周期蛋白 

分 类 号：R734.2[医药卫生—肿瘤]