气相色谱法测定大鼠血清脂肪酸  

Determination of free fatty acids in rat serum by gas chromatograph

作　　者：刘传华[1] 阎明[2] 刘贤锡[1] 孟繁立[2] 刘师莲[1] 丘蕾[3] 

机构地区：[1]山东大学医学院医学分子生物学实验中心 [2]齐鲁医院消化内科 [3]山东省立医院呼吸内科

出　　处：《山东大学学报（医学版）》2003年第1期70-71,共2页Journal of Shandong University:Health Sciences

基　　金：山东省科委科研基金资助课题(971226214)

摘　　要：目的:用气相色谱法测定大鼠酒精性脂肪肝血清中7种游离脂肪酸的含量。方法:选用10%丁二酸二乙二醇酯(DEGS)色谱柱,样品经乙酸乙酯提取后,用三氟化硼-乙醚/甲醇甲酯化处理。内标采用正十七碳酸。结果:月桂酸、豆蔻酸、硬脂酸与花生四烯酸的线性范围在1～50mg/L,棕榈酸、油酸与亚油酸的线性范围在10～100mg/L。7种脂肪酸的变异系数在0.9991～0.9998之间。7种脂肪酸的加样回收率在85.6%～94.3%之间。结论:本法简便、快速、可靠,为临床科学研究及疾病的诊断提供参考依据。Objective:To research on the practicability of gas chromatograph(GC)method for the de-termi nation of the seven free fatty acids in the serum of rat with alcoholic adipose liver.Meth ods:10%dethylenegly col succinate(DEGS)column was chosen and extractions with ethyl acetate from samples were methylated with BF 3 -ether /methanol.Re sults:The lin earity of C 12∶0 ,C 14∶0 ,,C 18∶0 ,and C 20∶4 ranged from1to50mg /ml ,and linearity of C 16∶0 ,C 18∶1 ,and C 18∶2 ranged from10to100mg /ml.The linear calibration coeffi-cients of the seven free acids were between0.9991～0.9998.The mean recovery of the sev en free fatty acids was85.6%～94.3%.Con clusion:This method was simple,speedy and reliable.The data obtained will be useful for clinical re search and di ag noses of diseases.

关 键 词：脂肪酸类 非酯化 色谱法 气相 脂肪肝 酒精性 大鼠 近交系 

分 类 号：Q95－343[生物学—动物学] R575.5[医药卫生—消化系统]