端粒酶基因hTRT在胃癌中的表达及其临床意义  

Expression of telomerase gene hTRT and its clinical significance in gastric cancer

作　　者：李贵新[1] 马世庆[2] 赵常在[2] 王会东[2] 石毅然[2] 徐功立[1] 

机构地区：[1]山东省立医院肿瘤研究治疗中心,济南250021 [2]山东省潍坊市人民医院肿瘤科

出　　处：《肿瘤》2003年第1期47-49,共3页Tumor

基　　金：山东省科学技术攻关计划基金资助(编号:981154503B)

摘　　要：目的 探讨胃癌中端粒酶基因hTRT mRNA表达与临床参数的关系,评价hTRT表达检测在胃癌诊断中的价值。方法用原位杂交的方法检测端粒酶基因hTRT mRNA在胃癌、癌旁组织和胃良性病变中的表达和分布情况。结果57例胃癌中hTRT基因表达阳性率为94.7％(54/57);57例癌旁组织中hTRT基因表达阳性率为0％(0/57);69例胃良性病变中hTRT基因表达阳性率为2.9％(2/69)。胃癌组织中HTRT基因的表达与癌旁组织、胃良性病变比较差异有显著性(P均<0.001)。胃癌组织中hTRT的表达与肿瘤分化程度、淋巴结转移相关(P均<0.05),但与肿瘤分期无相关性(P>0.05)。hTRT表达的阳性分级与淋巴结转移相关(P<0.01)。但与胃癌分化程度和分期无相关性(P均>0.05)。结论端粒酶基因hTRT表达检测在胃癌诊断中可能有一定的价值。Objective To investigate the correlation between the expressions of telomerase gene hTRT (human telomerase reverse transcriptase) and clinical parameters in gastric cancer, and to evaluate the role of detection of hTRT gene in gastric cancer diagnosis. Methods With in situ hybridization, the expression and distribution of telomerase gene hTRT was observed in 57 cases of gastric cancer tissues, 57 cases of tumor-adjacent tissues, 69 cases of benigh lesions.The results were statistically analyzed. Results hTRT was detected in 94.7%(54/57) of gastric cancer, 0%(0/57) in the adjacent tissues, and 2.9% (2/69) in benign lesions.The positive detection rate of hTRT expression in cancer group was significantly different from those in the adjacent tissues and benigh lesions group (both P<0.001). The expression of hTRT was correlated with degrees of differientiation and lymph node metastasis (P both<0.05),but not with tumor stage (P all>05). The expression level of hTRT was correlated with lymph node metastasis(P<0.01),but not with degrees of differientiation or tumor stage (P all>0.05). Conclusion The detection of telomerase hTRT gene expression may be useful in diagnosis in gastric cancer.

关 键 词：临床意义 胃癌 基因表达 HTRT基因 端粒酶 诊断 

分 类 号：R735.2[医药卫生—肿瘤]