IFN-α和TGF-β1对卵巢癌细胞SKOV3端粒酶活性的影响  

The effects of IFN-α and TGF-β1 on telomerase activity of ovarian cancer cell line SKOV3

作　　者：赵瑞皎[1] 李锦军[1] 葛超[1] 张锋锐[1] 薛鹏[1] 万大方[1] 顾健人[1] 

机构地区：[1]上海市肿瘤研究所癌基因及相关基因国家重点实验室,上海200032

出　　处：《肿瘤》2003年第2期124-128,共5页Tumor

基　　金：上海市卫生局青年科研基金 ( 1310 14Y17)

摘　　要：目的　观察IFN α和TGF β1对卵巢癌细胞系SKOV3端粒酶活性和凋亡的影响 ,探讨它们在肿瘤治疗中的作用机制。方法　用IFN α和TGF β1分别处理SKOV3细胞 ,然后采用端粒酶PCRELISA方法检测不同处理时间细胞的端粒酶活性表达 ,同时利用流式细胞仪观察处理前后SKOV3细胞的凋亡情况。用裸鼠体内成瘤实验及免疫组化 ,检测转染TGF β1基因对SKOV3细胞生长及端粒酶活性的影响。结果　IFN α对SKOV3细胞生长有抑制作用 ,可明显下调SKOV3细胞端粒酶活性 ;而TGF β1作用则相反 ,在体内外对SKOV3细胞生长均有促进作用 ,可明显上调SKOV3细胞端粒酶活性 ;IFN α和TGF β1均不诱导SKOV3细胞发生凋亡。结论　抑制端粒酶活性是IFN α抗卵巢癌治疗的一个重要机制 ;TGF β1在体内外对SKOV3细胞生长均有促进作用。Objective To observe the effects of IFN-α and TGF-β1 on telomerase activity and apoptosis of ovarian cancer SKOV3 cell line, and to explore their anti-cancer mechanism. Methods The changes of telomerase activity of SKOV3 cell line treated with IFN-α or /and TGF-β1 were detected by PCR ELISA method. Apoptosis was also determined by FCM method. The biological function of TGF-β1 on SKOV3 cell was studied by tumor formation assay in nude mouse and immunohistochemistry. Results IFN-α inhibited the growth of SKOV3 cell and down-regulated its telomerase activity. However, TGF-β1 increased the growth of SKOV3 cell and up-regulated its telomerase activity both in vitro and in vivo. Neither IFN-α nor TGF-β1 seemed to induce apoptosis in SKOV3 cell line. Conclusion That IFN-α inhibited telomerase activity in SKOV3 cell line is an important mechanism that may play a key role in the anti-tumor therapy for ovarian cancer. TGF-β1 can stimulate the growth of SKOV3 cells both in vitro and in vivo.

关 键 词：卵巢癌 干扰素-Α 转化生长因子-Β1 端粒酶 

分 类 号：R73－36[医药卫生—肿瘤] R737.31[医药卫生—临床医学]