胃黏膜病变演化过程中c-met基因表达改变及临床意义  

Detection of c-met Oncogene Mutation and Expression in Intestinal Type Gastric Carcinoma

作　　者：郭瑞芳[1] 石玉涛[1] 刘锦涛[2] 赵敏 吕有勇 

机构地区：[1]内蒙古自治区医院内一科,内蒙古呼和浩特010017 [2]广东省深圳市罗湖医院消化科,广东深圳518001 [3]北京市肿瘤研究所肿瘤分子生物学实验室,北京100034

出　　处：《内蒙古医学杂志》2003年第1期9-12,共4页Inner Mongolia Medical Journal

基　　金：内蒙古自治区卫生厅医药卫生科研计划项目项目号:3类 6 6号

摘　　要：目的 :研究胃黏膜病变演化及细胞癌变过程中c met基因表达改变及临床意义。方法 :采用PCR和PCR—SSCP方法检测c met原癌基因在肠型胃癌中的突变情况。利用组织微阵列技术对胃黏膜细胞癌变过程中c met基因的表达水平进行检测。结果 :以c met基因的 3个突变热点 17、18和 19外显子侧翼序列设计引物 ,扩增 32例肠型胃癌及癌旁组织标本 ,PCR—SSCP未检测到有异常突变带型。 15 4例胃镜活检组织标本中c met蛋白阳性表达在 30例浅表性胃炎为 6例 ,阳性率为 2 0 % ,在 33例萎缩性胃炎中有 8例 ,阳性率为 2 4 2 %。过表达率分别为 10 %和 12 1% ,两组间经统计检验P >0 0 5 ,无统计学意义。肠化及异型增生的阳性表达强度也主要为弱阳性及中等强度阳性 ,其中 4例中重度肠化生和 3例中重度及 1例轻度异型增生出现强阳性表达。 6例肠化和 6例异型增生为中等强度表达。c met阳性表达在 31例肠化中有 17例 ,阳性率为 5 4 8% ,在 30例异型增生中有 17例 ,阳性率为 5 6 7%。过表达率分别为 32 3%和33 3%。在胃癌主要是中等强度和强阳性表达。包括 6例中高分化腺癌 ,4例低分化腺癌 ,阳性率为5 3 3% ,过表达率 33 3%。肠化、异型增生和胃癌 3组间阳性表达率经统计检验P >0 0 5 ,无统计学意义。但 3者阳性率均明显高?Objective:To determine c met oncogene changes and clinical significance in intesinal type gastric carcinoma.Methods:We chose exon 17,exon18 and exon 19 of c met gene and detected their mutation in 32 tumor specimens of intestinal type gastric carcinoma by performing analysis of PCR and PCP-SSCP techiques.Expression of c met gene was examined by tissue micro array technique.The materials comprised 154 gastric biopsy specimens with superficial gastritis,chronic atrophic gastritis,intestinal metaplasia,dysplasia and carcinoma taken from a natural population at high risk for gastric cancer.Results:Our data demonstrated that none point mutation was detected in exon 17,exon 18 and exon 19 of c met gene.The accumulation rate of c met protein in superficial gastritis,chronic atrophic gastritis,intestinal metaplasia,dysplasia and carcinoma was respectively 20%,24.2%,54.8%,56.7% and 53.3%.The rate of overexpression was respectively 10%, 12 1% ,32.3%,33.3% and 33.3%.The accumulation rate of c met protein in intestinal metaplasia,dysplasia and carcinoma was significant higher than superficial gastritis and chronic atrophic gastritis(P<0.05).The rate overexpression of c met protein in intestinal metaplasia,dysplasia and carcinoma was higher than superficial gastritis and chronic atrophic gastritis(P>0.05).Conclusion:The result showed that the point mutation of c met gene is not the main pattern of alteration in intestinal type gastric carcinoma.The rate expression and over expression of c met protein in intestinal metaplasia,dysplasia and carcinoma was higher than that in superficial gastritis and chronic atrophic gastritis.Intestinal metaplasia may have a higher risk of human gastric cancer.Tissue micro array is a high throughput screening strategy for mutation detection and protein expression.

关 键 词：胃黏膜病变 C-MET基因 临床意义 胃癌 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]