3个桉树无性系过氧化氢酶活性及同工酶比较研究  

Comparative studies of catalase activities and isozymes in three clones of Eucalyptus

作　　者：黄卓烈[1] 詹福建[1] 巫光宏[1] 张东方[1] 赵赣[1] 谭绍满[2] 

机构地区：[1]华南农业大学生命科学学院,广东广州510642 [2]华南农业大学林学院,广东广州510642

出　　处：《亚热带植物科学》2003年第1期4-7,共4页Subtropical Plant Science

基　　金：广东省重点攻关项目(99MO4201G)内容的一部分

摘　　要：本文研究杂交桉树(Eucalyptus urophylla×Eucalyptus camaldulensis)LH21无性系、LH22无性系和尾叶桉(Eucalyptus urophylla)U6无性系的过氧化氢酶(CAT)活性及同工酶的差异。结果表明,3个桉树无性系同一器官的CAT活性存在差异,同一个无性系中不同器官的CAT活性差异很显著。采用聚丙烯酰胺不连续凝胶垂直板电泳比较分析CAT同工酶,发现3个无性系的CAT同工酶存在着一定的差异,其中LH21和LH22叶片有相同的谱带,但根的谱带与叶片有差异;而U6各器官的CAT谱带与LH21和LH22有差异。3个无性系的CAT同工酶都在一定程度上具有器官的特异性。Clones LH21, LH22 hybrid Eucalyptus (Eucalyptus urophylla×Eucalyptus camaldulensis) and clone U6 Eucalyptus urophlla were studied to compare the differences of catalase activities and the isoenzymes. The results indicated that catalase activity varied among the same organ of different clones, and significantly among the different organs of the same clone. LH21 and LH22 had the same catalase isoenzyme system in the leaves. But the catalase systems in the roots of LH21 and LH22 were different from those of the leaves and the stems. The catalase systems in U6 were also different from those of LH21 and LH22.

关 键 词：桉树 过氧化氢酶 活性 同工酶 

分 类 号：S792.39[农业科学—林木遗传育种] Q946.5[农业科学—林学]