生物衍生骨与成骨细胞联合培养体内异位成骨实验研究  

Research on ectopic osteogenesis in vivo by co-culture of bio-derived bone and osteoblasts

作　　者：陈文斌[1] 章庆国[1] 宋萍[2] 

机构地区：[1]东南大学附属中大医院整形外科,江苏南京210009 [2]东南大学基础医学院,江苏南京210009

出　　处：《东南大学学报（医学版）》2003年第2期101-104,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的 :探讨生物衍生骨作为组织工程骨支架复合成骨细胞体内植入的成骨作用 ,了解其用于骨组织工程支架材料的可行性。方法 :将经过物理化学方法处理制得的冻干猪脱蛋白型松质骨与胎兔颅骨来源的成骨细胞体外培养 10d后 ,在无菌条件下植入 3 0只成年新西兰兔背部肌肉内 (左侧 ) ,对照组为同体单纯植入猪脱蛋白型松质骨 (右侧 )。于 8周后取材 ,行大体观察、X线摄片、脱钙后组织学染色 (HE法 )及荧光标记检测 ,观察新骨形成情况。结果 :8周后 ,实验组 :X线片有高密度的阴影 ;大体标本呈红色 ,质硬 ;脱钙组织切片示大量的类骨质骨形成并相互连接成骨小梁结构 ,骨细胞位于陷窝中 ;荧光标记后在荧光显微镜下 ,骨小梁呈现淡绿色 ,类骨质呈黄色。对照组 :X线片仅见植入处低密度阻射阴影 ;组织学检查及荧光标记检测均无类骨形成 ,在支架的孔洞内有大量的纤维组织长入。结论 :猪脱蛋白型松质骨可作为骨组织工程的支架材料 。Objective To explore the osteogenic action of biological derivative bone as scaffolds of tissue engineering bone combined with osteoblast cultured in vitro and its applicability to act as scaffold materials of tissue engineering.Methods Porous heterogenous deproteinication bone (DPB)of pig obtained by physiochemical process and osteoblast acquired from the cranical bone of fetal rabbits were cultured 10 days in vitro and explanted into the left side of dorsa muscle of 30 adult rabbits.In control group,only DPB was explanted into the right side of dorsa muscle of the same adult rabbits.After 8 weeks,the specimen were extracted and observed for the formation of neonatal bone by gross inspection,X-rays,HE and fluorimetry.Results 8 weeks later,for the experimental group,the specimen were red and hard and X-rays showed shadow of high density.Slices stained by HE suggested formation of osteoid connected to be trabecula,and light fluorescence of trabecula was found in slices labelled with tetrayline.For the control group,shadow of low density was shown in X-rays.There was no formation of osteoid by histological and fluore scence detection,and a lot of fibrous tissue was found in the scaffold.Conclusion Deproteinization bone can be used as scaffolds of bone tissue engineering.

关 键 词：组织工程 成骨细胞 脱蛋白骨基质 兔 

分 类 号：Q813[生物学—生物工程] R－33[医药卫生]