抗黄矮病小麦易位系cDNA文库的构建  

CONSTRUCTION AND CHARACTERIZATION OF cDNA LIBRARY OF WHEAT TRANSLOCATION

作　　者：王晓萍[1] 郭东林[1] 徐淑红[1] 徐香玲[1] 李集临[2] 张增艳[2] 辛志勇[2] 

机构地区：[1]哈尔滨师范大学 [2]中国农业科学科学院作物所

出　　处：《哈尔滨师范大学自然科学学报》2003年第1期77-79,共3页Natural Science Journal of Harbin Normal University

基　　金：国家植物转因基专项(J99-A-011);"863"项目(2001AA222093)资助~~

摘　　要：对小麦-中间偃麦草易位系HW642一周龄幼苗接种饲黄矮病毒蚜虫,接种48 h后取幼叶提取mRNA构建了一个cDNA文库,文库的滴度为1.5×105pru/mL.随机挑取103个重组克隆,用限制内切酶EcoR I、Xho I消化,统计分析结果表明插入片段在150～4000 bp之间,平均插入片段大小为2000 bp左右,重组率为95.15 ％.文库可用于筛选抗病候选基因及EST研究等.A cDNA library of HW612 was constructed using mRNA isolated from wheat seedling leaf infected with aphids carrying barley yellow dwarf virus (RYDV) for 48hrs. The liter of the cDNA library is f. 5×106pfu/ml. 103 randomly selected recombinant clones were digested with EcoR I and Xho I to evaluate the cDNA library. The insert size of the cDNA library ranges from 150 to 4000bp with an average of 2000bp and the recombinant rate of the cDNA library is 95. 15%.

关 键 词：CDNA文库 小麦易位系 黄矮病 抗病基因 抗病机理 小麦病害 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治] Q785[农业科学—植物保护]