采用渗透交联固定化方法提高细菌内酶转化率的研究  

Studies on increasing turnover rate of endoenzyme of immobilized cells by permealilizing and crosslinking method

作　　者：金同立[1] 张培德[1] 

机构地区：[1]复旦大学生物化学系,上海200433

出　　处：《工业微生物》2003年第1期14-18,22,共6页Industrial Microbiology

摘　　要：采用渗透交联方法处理固定化棒状杆菌 ,并利用细胞内酶顺式环氧琥珀酸水解酶催化生产L(+ )酒石酸。确定了最佳渗透交联固定化条件 :多乙烯多胺浓度 1.2 5 % ,作用 30min ;戊二醛浓度 0 .75 % ,作用 15min。固定化细胞内酶转化的最适条件 :底物浓度为 0 .5～ 1.0mol L ;pH8.0 ;温度 37～ 4 2℃。结果发现 ,经渗透交联处理的固定化细胞酶活力比未处理的提高 5倍多。而且固定化细胞的颗粒硬度提高 10倍多 。Converting cis-epoxysuccinate to L(+) tartaric acid by immobilization cells of Corynebacterium producing cis-epoxysuccinate hydrolase was studied. The immobilization cells were treated with permealilizing and crosslinking methods. The optimum conditions were as follows: polyethylene-polyamine 1.25% for 30 min and glutardehyde 0.75% for 15 min; the optimum conditions of transfer cis-epoxysuccinate to L(+) tartaric acid by immobilized cells were as follows: pH 8.0 and 37～42℃. The concentration of sodium cis-epoxyuccinate was about 0.5～1.0 mol/L at pH 8.0 and 37℃. Average yield of L(+) tartaric acid produced by permealilized and crosslinked cells was more than five times compared with that of no permealilized and crosslinked cells; the tablet hardness of immobilization cells was more than ten times compared with that of no permealilizing and crosslinking cells; meanwhile the stability of immobilized cells was improved.

关 键 词：渗透交联 固定化 顺式环氧琥珀酸水解酶 通透性 酶工程 

分 类 号：Q939.9[生物学—微生物学]