EGF-PE35KDEL重组毒素的基因构建及表达  

Construction and Expression of Recombinant EGF-PE35KDEL Toxin Gene

作　　者：李树民[1] 李丽萍[1] 岳玉环[1] 李铁征[1] 邱业峰[1] 朱平[1] 

机构地区：[1]中国人民解放军军需大学军事兽医研究所,长春130062

出　　处：《中国生物制品学杂志》2003年第2期68-70,共3页Chinese Journal of Biologicals

摘　　要：目的　构建表达有特异细胞毒性融合蛋白的工程质粒。方法　利用PCR扩增出PE3 5KDEL基因片段 ,经酶切后插入EGF PET2 8A载体 ,并转化至BL2 1(DE3 )中。采用Westernblot对重组毒素进行鉴定。结果EGF PE3 5KDEL重组毒素占菌体总蛋白的 2 0 % ,分子量约为 410 0 0。结论　EGF PE3Objective To construct a engineering plasmid which can express special cytotoxic fusion protein.Methods Amplify PE35KDEL DNA fragment by PCR,digest with restriction endonuclease,insert into EGF PET28a vector and transform to BL21(DE3) for expression under induction of IPTG.The expressed product was identified by Western blot.Results The expressed EGF PE35KDEL toxin contained 20% of total somatic protein,and the relative molecular weight of it was about 41 000.Conclusion The successful construction and expression of EGF PE35KDEL laid a foundation for developing potential targeting antitumor medicine.

关 键 词：基因构建 表达 表皮生长因子 绿脓杆菌外毒素 重组毒素 

分 类 号：Q78[生物学—分子生物学]