养殖牙鲆鱼苗腹水症病原菌的鉴定及系统发育学分析  

IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF ONE PATHOGENIC BACTERIUM ASSOCIATED WITH SWOLLEN ABDOMEN OF CULTURED FLOUNDER (PARALICHTHYS OLIVACEUS) LARVAE

作　　者：莫照兰[1] 茅云翔[2] 陈师勇[1] 张振冬[1] 徐永立[1] 张培军[1] 

机构地区：[1]中国科学院海洋研究所 [2]中国海洋大学海洋生命学院,青岛266003

出　　处：《海洋与湖沼》2003年第2期131-141,共11页Oceanologia Et Limnologia Sinica

基　　金：国家重点基础研究发展规划项目 ( 973 )专项资助 G1 9990 1 2 0 0 3号;国家高技术研究发展计划项目资助 ( 863 )2 0 0 1AA62 0 2 0 7号

摘　　要：从患腹水症养殖牙鲆鱼苗分离到一株细菌B1 7,人工感染实验证实其具有致病性。用Biolog GN细菌鉴定系统对B1 7进行测试 ,发现其与参考菌株的相似性较低 ,不能进行鉴定。常规生理生化测试表明 ,B1 7具有弧菌属的特征 ,其表型特征与Vibriosplendidus非常相似。为了进一步明确菌株B1 7的分类学地位 ,测定了其 1 6SrRNA基因序列 ,分析了相关细菌相应序列的同源性 ,构建了系统发生树 ,结果表明菌株B1 7与V .splendidus的亲缘关系最近。综合上述结果 ,菌株B1 7可鉴定为V .splendidus。Bacterial strain B17, which was isolated from the Japanese flounder ( Paralichthys olivaceus ) larvae with swollen abdomen, was proved to be a pathogenic organism by oral infection experiments. Biolog GN (gram negative) identification system were applied in the determination of strain B17, showing 65.7% similarity with reference strain of Vibiro vulnificus. The similarity was low and could not give identification of strain B17. The traditional physiological and biochemical methods showed that most of the phenotypes of strain B17 were similar to Vibiro splendidus, according to the description of Paul et al (1984). In order to investigate the phylogenetic position of this pathogen, a 1477bp of 16S rRNA gene was sequenced and compared with that of related strains. Molecular phylogenetic dendrogram was constructed based on the genetic distance analysis, which revealed that strain B17 exhibited the highest levels of 99.3% similarity to Vibiro splendidus. Based on the results obtained from phenotypes and 16S rRNA gene analysis, strain B17 was identified as Vibiro splendidus .

关 键 词：牙鲆 腹水症 16SRRNA基因 系统发育学分析 Vibriosplendidus 

分 类 号：Q789[生物学—分子生物学]