淋巴细胞与肝癌细胞混合培养诱导特异性细胞毒T淋巴细胞的形成  

Co-culture of lymphocytes and hepatocellular carcinoma cells induces specific cytotoxic T-lymphocyte lines

作　　者：李均乐[1] 叶小鸣[2] 程权永[2] 陈祖兵[2] 王昌俊[1] 张华[1] 李湘宏[2] 田立新[1] 杨振淮[1] 

机构地区：[1]广州市中医院,510130 [2]中山大学附一医院

出　　处：《中华普通外科杂志》2003年第3期139-140,共2页Chinese Journal of General Surgery

摘　　要：目的通过肝癌细胞和淋巴细胞混合培养 ,体外诱导产生高活性的肝癌特异性细胞T淋巴细胞 (H S CTL)。方法采用淋巴细胞和肝癌细胞混合培养技术 ,在IL 1,IL 2 ,IL 4和IL 6刺激下 ,诱导产生H S CTL ,应用间接免疫荧光法和51Cr释放法检测其对靶细胞的杀伤效应。结果H S CTL与自身LAK相比 ,CD3+,CD4+和CD8+细胞明显增多 ,抗肿瘤效应明显增强。结论采用淋巴细胞和肝癌细胞混合培养同时应用白细胞介素可获得大量扩增的高活性的H S CTL。Objective To induce highly active hepatocellular carcinoma (HCC) specific cytotoxic T-lymphocyte (H-S-CTL) lines by co-culture of lymphocytes and hepatocellular carcinoma cells. Methods H-S-CTL were established by co-culture of autologous HCC cells and lymphocytes, under stimulation of IL-1,IL-2,IL-4 and IL-6.The cytotoxic activity of H-S-CTL for target cells were detected by 51Cr release assay and indirect immunofluorescence assay. Results Compared with auto-LAK, H-S-CTL showed much higher antitumor activity of significantly increased CD3+, CD4+ and CD8+ cells. Conclusions H-S-CTL lines with massive proliferations and high antitumor activity can be successfully obtained by ex vivo co-culture under the stimulation of interlukins.

关 键 词：淋巴细胞 肝癌细胞 混合培养 特异性细胞毒T淋巴细胞 

分 类 号：R735.7[医药卫生—肿瘤]