乳腺癌患者B7-CD28共刺激通路的研究  

A study on the B7-CD28 costimulatory pathway in breast cancer patients

作　　者：范萍[1] 王水[1] 刘晓[1] 武正炎[1] 柴伟栋[2] 

机构地区：[1]南京医科大学第一附属医院普外科,210029 [2]南京医科大学第一附属医院内分泌科,210029

出　　处：《中华普通外科杂志》2003年第3期162-164,共3页Chinese Journal of General Surgery

基　　金：江苏省教委自然科学基金资助 ( 0 1KJD32 0 0 1)

摘　　要：目的探讨乳腺癌患者外周血中CD2 8表达阳性的T细胞数量和患者乳腺癌原代细胞表面B7 1(CD80 )、B7 2 (CD86 )的表达水平 ,以探讨乳腺癌患者共刺激通路是否异常。方法采用流式细胞技术检测乳腺癌患者外周血中CD2 8阳性T细胞与乳腺癌患者原代细胞B7分子的表达 ,并与乳腺良性疾病作比较。结果乳腺癌患者CD2 8阳性的T细胞数明显低于对照组 (t =2 879,P <0 0 5 ) ,特别是CD4/CD2 8双阳性T细胞数更低于对照组 (t=3 0 17,P <0 0 0 1)。乳腺癌患者原代细胞表面B7 1和B7 2表达水平都低于对照组。结论乳腺癌患者外周血中T细胞低表达CD2 8分子 ,乳腺癌细胞低表达B7分子 ,从而影响B7 CD2 8共刺激通路 ,使T细胞不能有效地清除肿瘤细胞。Objective To investigate costimulatory pathway of breast cancer patients, we examined peripheral CD28 positive T lymphocytes and B7 molecules expression on the primary breast cancer cells in patients with breast cancer. MethodsFlow cytometry was used to analyze CD28 and B7 molecules expressed on T lymphocytes and primary breast cancer cells respectively. Results CD28 positive T lymphocytes in breast cancer patients was lower than that in control group (t=2.879,P<0.05), with CD4/CD28 double positive T lymphocytes being much lower (t=3.017,P<0.005). Both B7-1(CD80) and B7-2(CD86) molecules expressed on primary breast cancer cells were lower than that in control group. ConclusionPeripheral T lymphocytes in breast cancer patients express low level of CD28 molecule. Primary breast cancer cells express low level of B7 molecules. Costimulatory pathway dysfunction in breast cancer patients results in T lymphocytes failure against the tumor cells.

关 键 词：乳腺癌 B7-CD28 共刺激通路 研究 

分 类 号：R737.9[医药卫生—肿瘤]