前列腺癌患者外周血PSA mRNA的意义  

Detection of PSA mRNA in peripheral blood from patients with prostate cancer

作　　者：刘向袆[1] 杨凌[1] 毕维琪[2] 

机构地区：[1]首都医科大学附属北京同仁医院临床检测中心,北京100730 [2]首都医科大学附属北京同仁医院泌尿外科,北京100730

出　　处：《临床检验杂志》2003年第2期78-79,共2页Chinese Journal of Clinical Laboratory Science

摘　　要：目的　探讨前列腺特异抗原 (PSA)mRNA在前列腺癌 (PCa)患者外周血表达的意义。方法　运用巢式RT PCR检测 36例PCa患者 ,2 8例良性前列腺组织增生 (BPH)患者和 15例健康志愿者外周血。结果　BPH患者和志愿者外周血PSAmRNA均阴性 ,36例PCa外周血PSAmRNA阳性率 38 9% (14 / 36 )。PSAmRNA表达阳性率随肿瘤分期进展而增高 ,各期阳性率有显著性差异 (P <0 0 5 )。结论　PSAmRNA特异表达于PCa外周血 。Objective To assess the clinical significance of detection for prostate specific antigen (PSA) mRNA in peripheral blood from the patients with prostate cancer.Methods PSA mRNA expression in 36 prostate cancer patients, in 28 benign prostate hyperplasia (BPH) patients and 15 heathly volunteers was detected by nested RT PCR.Results PSA mRNA expression was undetectable in BPH patients and healthy volunteers while positive expression was observed in 14 of prostate cancer patients (38.9%). The positive percentage of PSA mRNA expression increased with stages of tumor and significant differences were found in various stages( P <0.05).Conclusion PSA mRNA specifically expresses in peripheral blood from prostate cancer patients and may be a clinically useful marker for micrometastasis of prostate cancer.

关 键 词：前列腺癌 外周血 MRNA 前列腺特异抗原 逆转录聚合酶链反应 微转移 临床意义 

分 类 号：R737.25[医药卫生—肿瘤]