大鼠脊髓内5-HT_(2C),5-HT_3,5-HT_6和5-HT_7受体亚型mRNAs的表达(英文)  

Expression of mRNAs encoding for 5-HT_(2C) , 5-HT_3 , 5-HT_6 and 5-HT_7 receptor subtypes in rat spinal cord

作　　者：武胜昔[1] 王亚云[1] 刘翔宇[1] 王文[1] 李云庆[1] 

机构地区：[1]第四军医大学基础部解剖学教研室暨梁銶琚脑研究中心,陕西西安710032

出　　处：《中国神经科学杂志》2003年第2期69-73,共5页

基　　金：国家自然科学基金 (3 9970 2 3 9;3 980 0 0 3 4) ;高等院校骨干教师资助计划资助课题 ;国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G19990 5 40 0 4)项目资助

摘　　要：目的 :观察 5 HT2C,5 HT3,5 HT6 和 5 HT7受体亚型mRNAs在大鼠脊髓不同节段的表达。 方法 :反转录PCR方法。 结果 :5 HT2C受体亚型mRNA在颈、胸、腰、骶段脊髓的背角 (DH)和前角 (VH)均有较强的表达 ;5 HT3受体mRNA在各节段脊髓DH的表达水平较高 ,而在VH则较低 ;与 5 HT3受体亚型相反 ,5 HT6 受体亚型mRNA在脊髓VH的表达水平高于DH ;5 HT7受体mRNA在脊髓的表达则与 5 HT3受体相似 ,在各节段的DH均有较高水平的表达。不同的受体亚型在脊髓同一节段以及同一受体亚型在不同脊髓节段的表达水平存在差异。结论 :本研究结果表明 ,上述四种 5 HT受体亚型在脊髓具有不同的表达特点 ,提示它们在脊髓水平可能发挥着不同的生理作用 ,并为深入探讨 5 HT受体参与伤害性感受和运动的调节机制提供了依据。Objective:To examine the expression of mRNAs encoding for serotonin (5 HT) 5 HT 2C , 5 HT 3 , 5 HT 6 and 5 HT 7 receptor subtypes within different segments of the rat spinal cord. Methods: Reverse transcriptase polymerase chain reaction (RT PCR) technique was used. Results: Strong expression of 5 HT 2C receptor mRNA was observed in both dorsal horn (DH) and ventral horn (VH) of the cervical, thoracic, lumbar and sacral segments of the spinal cord. The 5 HT 3 receptor mRNA was present at high expression level in the DH and at slightly lower expression level in the VH of all spinal cord segments. In contrast, the VH generally contained higher expression level of 5 HT 6 receptor subtype mRNA when compared to the DH. Similar to 5 HT 3 receptor, the 5 HT 7 receptor mRNA was also found at high expression level in the DH. The differences in the expression level among these 5 HT receptor subtypes at the same level of the spinal cord or the same receptor subtype in different segments of the spinal cord were also observed. Conclusion: Four serotonin receptor subtypes show a distinct expression pattern in the spinal cord. The present results indicate that these 5 HT receptor subtypes might have different physiological roles at the spinal level and provide further evidence for 5 HT receptor underlying the mechanism of nociception and movement.

关 键 词：大鼠 脊髓 5-HT2C 5-HT3 5-HT6 5-HT7 受体亚型 MRNAS 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]