双功能抗体介导结肠癌细胞杀伤效应的研究  

Bispecific McAb Mediated Tumor Growth Inhibition in Colonic Cancer

作　　者：高学硕[1] 万文徽[1] 张宏[1] 

机构地区：[1]北京医科大学临床肿瘤学院北京市肿瘤防治研究所北京肿瘤医院检验科,北京市100036

出　　处：《中国肿瘤临床》2003年第3期214-216,共3页Chinese Journal of Clinical Oncology

基　　金：北京市自然科学基金项目资助(7932002)

摘　　要：目的:研究抗CD3/抗TNP双功能抗体与TNP化抗癌胚抗原(CEA)McAb结合,在裸鼠体内对结肠癌细胞的杀伤作用。方法:将抗癌胚抗原单抗TNP化,TNP化后的抗CEA单抗(TNP-CEA)、抗CD3/抗TNP双功能抗体、人淋巴细胞及结肠癌细胞CL187接种于裸鼠体内,计算肿瘤生长体积,观察成瘤率。结果:双功能抗体能够介导PBLs对CL187细胞的杀伤作用。结肠癌细胞CL187的成瘤能力受到抑制,成瘤率为0/4。结论:此双功能抗体在免疫治疗中具有潜在的应用价值。Objective:To study in vivo the effects of the bispecific antibody(BsAb)com-bined with TNP-CEA to kill the colonic cells in nude mice.Methods:The anti-CEA antibody was crosslinked with TNP.TNP-CEA、anti-CD 3 /anti-TNP、PBLs and CL187were injected into nudemice to proceeded with tumor growth inhibition test in vivo and to observed the formed tu-mor ratio.Results:The bispecific antibody could induce P BLs to cytotoxic test of colonic cells in vivo.Tumor growth was inbibited and formed tumor ratio was0/4.Conclusion:The bispecific McAb has potential Value in the immuno-biotherapy of tumors.

关 键 词：结肠肿瘤 细胞系 双功能抗体 三硝基苯 

分 类 号：R735.35[医药卫生—肿瘤]