人喉癌组织中p15、p16基因缺失和STK15基因过表达的研究  

Deletion of p15 and p16 genes and overexpression of STK15 gene in human laryngeal squamous cell carcinoma

作　　者：李福才[1] 李英慧[1] 赵旭[1] 康宁[1] 富伟能[1] 徐振明 李增刚 孙开来[1] 

机构地区：[1]中国医科大学医学遗传学教研室,沈阳110001 [2]空军第四六三医院耳鼻喉科 [3]国家新药安全评价研究中心

出　　处：《中华医学杂志》2003年第4期316-319,共4页National Medical Journal of China

基　　金：国家自然科学基金(30171008);辽宁省自然科学基金(2001101039)

摘　　要：目的 探讨p15、p16基因和STK(丝氨酸/苏氨酸激酶)15基因表达与喉癌发生、发展的关系。方法 自50例术前未经化疗和放疗的喉鳞状细胞癌患者的新鲜手术标本中,分别取癌组织和癌旁正常组织进行以下检测:(1)提取DNA,采用聚合酶链反应(PCR)技术,进行p15基因第二外显子(p15E2)和p16基因第二外显子(p16E2)纯合缺失研究;(2)提取RNA,反转录合成cDNA,以β-肌动蛋白基因为内对照进行PCR扩增,分析STK15基因表达的水平。同时检测人喉鳞状细胞癌Hep-2细胞系STK15基因表达水平。结果 喉癌组织p15E2纯合缺失率为12％(6/50),p16E2纯合缺失率为14％(7/50),p15E2、p16E2共同缺失率为6％(3/50)。50例患者中34例(68％)癌组织STK15基因表达高于癌旁正常组织;全组患者癌组织STK15基因表达/β-肌动蛋白基因表达平均密度比值(ADV)为1.03±0.30,癌旁正常组织STK15基因表达/β-肌动蛋白基因表达ADV为0.89±0.22,其间差异有非常显著意义(t=4.333,P<0.01)。Hep-2细胞系中STK15基因表达高于内对照β-肌动蛋白基因。结论p15E2、p16E2纯合缺失和STK15基因过表达可能在喉癌的发生及恶性进展中发挥一定作用。Objective To investigate the association of p15 and p16 genes deletion, and STK15 gene overexpression with laryngeal squamous cell carcinoma ( LSCC) . Methods The cancer tissue and surrounding normal tissue were taken during operation from 50 cases of LSCC who had undergone neither chemotherapy nor radiotherapy preoperatively. DNA was extracted and PCR was used to test the homozygous deletion of p15 exon 2 (p15E2) and p16 exon 2 (p16E2) . RNA was extracted, cDNA was synthesized by reverse transcription, and the expression of STK15 gene was tested by PCR with β-actin as inner control. At the same time the expression of STK15 in human LSCC Hep-2 cell line was tested. The ratio of ADV (average density value) of STK15 gene to the ADV of β-actin gene was calculated. Results The rate of p15E2 deletion was 12% (6/50) and that of p16E2 was 14% (7/50). The p15E2 and p16E2 codeletion rate was 6% (3/50) . In 34 of the 50 cases (68%) the expression of STK15 gene in tumor tissue was higher than that of the paired surrounding normal tissue with a significant difference. The ratio of ADV of STK15 gene to ADV of β-actin gene was 1.03 β 0.30 in the cancer tissue, and 0.89 β 0.22 in the paired normal tissue with a significant difference ( t = 4.333, P < 0.01) . The expression of STK15 gene was higher than that of β-actin in Hep-2 cell line. Conclusion The homozygous deletion of p15E2 and p16E2 and overexpression of STK15 gene may play a role in the oncogenesis and malignant progression of laryngeal squamous cell carcinoma.

关 键 词：喉癌 P15 P16 基因缺失 STK15基因 过表达 

分 类 号：R739.62[医药卫生—肿瘤]