微囊化大鼠胰岛异种移植的实验研究  

The experimental study of microencapsulated islets xenotransplantation

作　　者：王国兴[1] 张洪德[2] 王煜非[2] 董维平[2] 王丽娟[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院内分泌科,杭州310016 [2]上海市第一人民医院糖尿病研究室

出　　处：《中华器官移植杂志》2003年第2期89-91,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　通过动物实验明确微囊化胰岛是否具有免疫隔离作用。方法　SD大鼠胰腺原位消化 ,Ficoll间断密度梯度离心法纯化、分离胰岛 ,气流吹喷制作海藻酸钠 /聚赖氨酸 /海藻酸钠(APA)微囊化大鼠胰岛 ,比较微囊化与未微囊化胰岛的胰岛素释放试验 ;将微囊化 (实验组 )与未微囊化 (对照组 )大鼠胰岛植入链脲佐菌素 (STZ)诱导的I型糖尿病小鼠中 ,作两组间血糖正常持续时间比较。结果　实验组与对照组的胰岛素释放试验差异无显著性 (P >0 .0 5 ) ;实验组血糖正常持续时间为 2 3～ 6 5d(平均 48d) ,对照组为 3～ 6d(平均 5d) ,两组差异有极显著性 (P <0 .0 1)。已排斥的实验组小鼠腹腔灌洗发现部分微囊化胰岛存活 ,部分已坏死 ,但微囊膜皆完整 ,囊壁无纤维化。结论　微囊具有良好的免疫隔离作用 ,可使胰岛移植物存活时间明显延长。同时推测微囊内移植物死亡与细胞因子、自由基作用或营养不足等有关。Objective To affirm whether microencapsulated islets have immunoisolated effect by animal experiments.Methods SD rats' islets were isolated by digestion in-situ and purified by Ficoll's discontinuous density gradient centrifugation. Then the islets were microencapsulated with alginate/poly-L-lysine/alginate (APA) by an air-jet nozzle. The insulin release tests of microencapsulated and free islets were contrasted. The microencapsulated (experimental group) and free islets (control group) were xenotransplated into streptozotocin-induced type 1 diabetic mice intraperitoneally, and the duration of normoglycemia was contrasted. Results There was no significant difference in insulin release function between microencapsulated and free islets (P> 0.05). The duration of normoglycemia in experimental group was 23～65 days (mean 48 days), while 3～6 days (mean 5 days) in control group, with the difference being very significant between the two groups (P< 0.01). Peritoneal cavity's washing tests of the rejected experimental group showed partial islets in microcapsules were still alive, others died, but all microcapsule members were intact without any wall fibrosis.Conclusions Microcapsules have fine immunoisolated effect, and could obviously prolong the survival of islets graft. At same time it could be conferred that the death of grafts in microcapsules may be due to the effect of cytokine, free radicals or malnutrition and so on.

关 键 词：微囊化 大鼠 胰岛异种移植 实验研究 免疫隔离 

分 类 号：R657.5[医药卫生—外科学]