转化生长因子β-1在全脑缺血再灌注后迟发性神经元坏死时的表达  

To protect and repair delayed neuronal death after global ischemia and reperfusion

作　　者：李华军[1] 尹姣[2] 陈东[3] 李巍[4] 邵延坤[1] 田沈 王哲[1] 饶明俐[1] 

机构地区：[1]吉林大学第一医院,吉林长春130021 [2]北京空军总医院,北京100036 [3]吉林大学基础医学部,吉林长春130021 [4]北华大学附属医院,吉林吉林132001 [5]沈阳铁路中心医院,辽宁沈阳110006

出　　处：《中风与神经疾病杂志》2003年第1期52-54,共3页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　探讨转化生长因子β- 1(TGF-β1 )在迟发性神经元坏死时对中枢神经系统的保护与修复作用。方法　采用 HE染色、尼氏染色、透射电镜、免疫组化及 RT- PCR技术。结果　常规光镜及电镜观察均显示再灌注后额叶皮质及海马有不同程度的神经元变性、坏死。免疫组织化学结果及 RT- PCR结果均显示皮质与海马区内的TGF-β1 在缺血再灌注后呈明显阳性改变。结论　 TGF-β1 在脑缺血损伤时尤其在发生迟发性神经元坏死时可呈现明显强于正常脑组织的表达 ,因此 ,TGF-β1 不仅在脑缺血早期而且在发生迟发性神经元坏死时 。Objective To research the mobile changes of cell factor TGFβ 1 expression and the role in protect and repair function in CNS when delayed neuronal death. Methods Using HE stains,Niles stains,transmission electron microscope,immunohistochemistry and RT PCR technique etc. Results Light and electron microscope can both find the varying degrees neural denaturation and necrosis after ischemia reperfusion,immunohistochemistry and RT PCR examine show that TGFβ 1 in cortex and hippocamal tissue significantly positive expressed. Conclusion TGFβ 1 significantly positive expressed when cerebral ischemia injure,specially while delayed neuronal death,it may play an important roles in repair and protective function for CNS.

关 键 词：小鼠 脑缺血 再灌注 迟发性神经元坏死 转化生长因子Β-1 

分 类 号：R743.31[医药卫生—神经病学与精神病学]