胃癌MAGE-1基因启动子B′B区去甲基化状态的研究  

Methylation State within MAGE-1 B′B Promoter in Gastric Carcinoma

作　　者：许琳[1] 罗兵[2] 王青[1] 徐翮飞[2] 黄维青[1] 梁华[2] 

机构地区：[1]青岛市立医院,山东青岛266011 [2]青岛大学医学院微生物教研室,山东青岛266011

出　　处：《中国肿瘤生物治疗杂志》2003年第1期39-41,共3页Chinese Journal of Cancer Biotherapy

摘　　要：目的 :探讨胃癌组织中MAGE 1基因启动子B′B区去甲基化状态及其与病理分级及临床分期的关系。方法 :取胃癌组织标本 4 0例 ,另外取同患者相应的癌旁组织 4 0例作为对照。采用分子生物学技术 甲基化敏感性内切酶酶切及PCR扩增技术 ,研究了胃癌组织中MAGE 1启动子B′B区的去甲基化状态。结果 :在所检测的胃癌组织标本中MAGE 1基因启动子B′B区去甲基化的发生率为 2 5 % (10 /40 )。而在癌旁组织中发生率为 0 ,两者发生率的差别具有显著的统计学意义 (P <0 0 1)。在低分化腺癌中MAGE 1基因的B′B区去甲化发生率为 5 0 % (6 /12 ) ,中分化腺癌中发生率为 18.7% (3/16 ) ,高分化腺癌中发生率为 8.3(1/11)。其发生率的差异有统计学意义 (P <0 .0 5 )。在早期胃癌组织中B′B区的去甲基化的发生率为16 .7% ,在晚期胃癌组织中发生率为 2 8.6 % (P <0 .0 5 ) ,差别有统计学意义。结论 :胃癌组织中MAGE 1基因启动子的B′B区存在去甲基化。Objective: To study the methylation state within MAGE 1 B′B promoter in gastric carcinoma and the association between demethylation and pathological differentiation, the association between demethylation and clinical stage. Methods: Using methylation sensitive restriction analysis followed by polymerase chain reaction (PCR),we studied 80 specimen that were obtained from surgical samples (including 40 gastric carcinoma and 40 matched adjacent normal gastric mucosae).Results: An demethylation state was identified in DNA from gastric carcinoma specimens.The demethvlation rate is 25%(10/40).In contrast,no demethylation state was identified in DNA from matched adjacent normal gastric mucosae. The differences were Significant statistically. ln our study, the demethylation in poorly, moderately, and well differentiated glandulous cell carcinoma were detected at frequencies of 50%,18.7% and 8.3%,respectively, The differences were significant statistically ( P <0.05). The demethylation rate in early, 1ate tumor stage was 16.7% and 28 6%,respectively. The differences were significant statistically( P <0.05). Conclusions: The demethylation in MAGE 1 B′B promoter is anomalous in gastric carcinoma, and maybe it is related to the expression of MAGE 1 gene in gastric carcinoma. The demethylation rate is related to the pathological and disease stages differentiation.

关 键 词：胃癌 MAGE-1基因启动子 去甲基化 

分 类 号：R735.2[医药卫生—肿瘤]