Ad-wtp53对p53状态不同结直肠癌细胞生长的影响  

The Effects of Recombinant Adenovirus-Mediated Wild Type p53 cDNA on Human Colorectal Cancer Cell Lines with Different p53 Status

作　　者：阎昭[1] 李雯[1] 牛瑞芳[1] 史玉荣[1] 郝希山[1] 

机构地区：[1]天津医科大学肿瘤医院,天津300060

出　　处：《中国肿瘤生物治疗杂志》2003年第1期42-47,共6页Chinese Journal of Cancer Biotherapy

摘　　要：目的 :探索重组腺病毒介导的外源性野生型 p5 3cDNA(Ad wtp5 3)对内源性 p5 3基因状态不同结直肠癌细胞的抑制作用是否存在差异 ,研究 p5 3基因抑制肿瘤细胞增殖的机制。 方法 :采用MTT法选择Ad wtp5 3的最佳转染滴度 ,分别感染内源性 p5 3基因缺失、突变和正常的 3种结直肠癌细胞株 ,观察比较它们的生长抑制作用。 结果 :Ad wtp5 3在 10 0 0MOI时表现出最强的细胞抑制作用 ,p5 3缺失细胞株 (THC 890 8)的抑制效应最明显 ,3种细胞转染后均发生G0 /G1期阻滞 ,并对不同p5 3状态细胞G2 M期具有不同的调控作用。结论 :外源性野生型p5 3cDNA导入可显著抑制结直肠癌细胞生长 ,使细胞周期停滞于G0 /G1期 ;同时对内源性 p5 3状态不同细胞的生长抑制强度和G2Objective: To explore the inhibition effects of ectogenic wild type p53 cDNA(Ad wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor. Methods: MTT process was taken to choose optimal transfection titre. Three kinds of cell lines(p53 gene deletion, mutation and nomal) were transferred by Ad wtp53 in optimal titre. The inhibition effects of these cell lines were observed and compared. Results: The best titre is 1000 MOI and p53 gene deletion cell line (THC 8908) shew the highest sensitivity. G 1 S transition period blocking effects occurred in all cell lines and G 2 M phase regulation effects were not coincidence in three colorectal cell lines. Conclusions: Recombinant adenovirus mediated wild type p53 gene observably inhibited colorectal carcinoma cell lines growth and proliferation, blocked cell cycle in G 0 /G 1 phase and displayed obvious different actions on G 2 M phase among cell lines with different p53 status.

关 键 词：基因治疗 P53基因 腺病毒载体 结直肠癌 

分 类 号：R735.34[医药卫生—肿瘤]