螅状独缩虫(Carchesium polypinum)类中间纤维的初步研究  

The Evidence for the Existence of Intermediate-like Filaments in Carchesium polypinum

作　　者：胡国群[1] 高崇明[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《北京大学学报（自然科学版）》2003年第2期178-181,共4页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金资助项目(39730070)

摘　　要：利用间接免疫荧光和免疫印迹发现螅状独缩虫中存在两种分子质量为 5 8kD和 66kD的蛋白。这两种蛋白能分别与抗波形蛋白和抗核纤层蛋白B的抗体反应。分子质量 66kD的蛋白位于大核边缘。另外 ,细胞经分级抽提后发现在大核周围存在核纤层样结构。基于上述结果 ,可以认为螅状独缩虫中存在类中间纤维。Using immuno-fluorescence labeling and immunoblot techniques,two intermediate filament-like proteins were identified in Carchesium polypinum .The molecular weights of these two proteins are 58?kD and 66?kD respectively.The 58?kD protein reacts with anti-vimentin antibody and locates near the aboral wreath cilia.The other protein reacts with anti-lamin B antibody and is situated in the macronucleus.After the sequential fraction treatment,a nuclear lamina-like layer can be seen surrounding the macronucleus.Based on these results,it is concluded that intermediate-like filaments exist in Carchesium polypinum .

关 键 词：螅状独缩虫 类中间纤维 核纤层 波形蛋白 核纤层蛋白B 

分 类 号：Q26[生物学—细胞生物学]