黑环蜂和黄环蜂各品系基因组DNA的多态性分析  

Genomic DNA Polymorphic Analysis in Strains of Black-ring Bee and Yellow-ring Bee

作　　者：邬珺超[1] 蒋滢[1] 薛运波[2] 葛凤晨[2] 

机构地区：[1]苏州大学医学院,江苏苏州215007 [2]吉林省养蜂科学研究所,吉林丰满131208

出　　处：《蜜蜂杂志》2003年第4期7-9,共3页Journal of Bee

基　　金：江苏省自然科学基金课题(BK2 0 0 00 2 6)

摘　　要：应用随机引物 (W ,5 CGGCCCCGGT 3 ;K ,5 CGGCCCCTGT 3 ;P ,5 ACCCAGGTTG 3 )分别对黑环蜂 3个品系和黄环蜂 4个品系的DNA基因组进行PCR扩增 ,结果获得不同数量的条带和相对分子质量不同的多态DNA图谱 ,从而为区分黑环蜂 (卡尼鄂拉蜂、珲春黑蜂、东北黑蜂 )和黄环蜂 (黄环系蜂、老美意蜂、浙农大意蜂、平湖意蜂 )random primers(W,5 CGGCCCCGGT 3;K,5 CGGCCCCTGT 3;P,5 ACCCAGGTTG 3)and PCR were used to amplify the genomic DNA in strains of black ring bee(Carniolian bee,Huichun black bee,Northeast black bee)and yellow ring bee(Yellow ring bee,American Italian bee,Zhejiang Agriculture University Italian bee,Pinghu Italian bee).The results showed that all products of amplification contained multiple brands but only the polymorphic DNA maps which got from amplification with P primer could help us to identify the strains of black ring bee and yellow ring bee respectively.

关 键 词：基因组 DNA 多态性分析 黑环蜂 黄环蜂 品系 RAPD 蜜蜂 

分 类 号：S891.2[农业科学—特种经济动物饲养]