低剂量^(131)I标记的抗癌胚抗原抗体C50联合5-FU对结直肠癌裸鼠移植瘤生长的影响  

Influence of Combination of Low Dosage ^(131)I labeled Anti-Carcinoembryonic Antigen Antibody C50 and 5-Fluorouracil on Tumor Growth of Colorectal Cancer Xenografts in Nude Mice

作　　者：郑朝旭[1] 詹文华[1] 蔡世荣[1] 何裕隆[1] 林展江[2] 

机构地区：[1]中山大学附属第一医院普通外科,广东广州510080 [2]中国广州分析测试中心,广东广州510070

出　　处：《癌症》2003年第4期354-357,共4页Chinese Journal of Cancer

基　　金：中山大学"211工程"重点学科建设基金资助项目(No.98097)

摘　　要：背景与目的:癌胚抗原(CEA)在结直肠癌组织中表达率高达90%以上,本研究拟探讨低剂量131I标记的抗CEA单抗C50(131I-C50)及低剂量131I-C50联合5-氟尿嘧啶(5-FU)对人结直肠癌移植瘤生长的影响。方法:建立表达CEA的人LoVo结直肠癌裸鼠移植瘤模型。接种第9天,分别采用5-FU、2775kBq131I-C50及5-FU联合131I-C50尾静脉注射治疗荷瘤裸鼠。尾静脉给药后第7天,每组各随机处死2只荷瘤裸鼠,观察肿瘤细胞超微结构改变及组织病理学改变。计算各组裸鼠肿瘤体积、群体倍增时间及抑瘤率,比较接种第30天的肿瘤体积。结果:对照组、5-FU组、131I-C50治疗组和131I-C50联合5-FU组接种第30天肿瘤体积分别为(9765.19±792.21)mm3、(6533.75±601.14)mm3、(5413.57±415.46)mm3和(3865.23±263.57)mm3,四组之间差异均有显著性(P<0.001);四组肿瘤群体倍增时间依次延长,分别为3.07、3.09、3.18和3.14天;后三组抑瘤率依次增大,分别为30.97%、42.33%和59.04%。结论:低剂量131I-C50可抑制结直肠癌裸鼠移植瘤的生长,低剂量131I-C50联合5-FU可以增强抑制肿瘤生长的效果。BACKGROUND ＆OBJECTIVE: The aim of this study was to investigate the influence of low dosage 131I labeled anti carcinoembryonic antigen (CEA) monoclonal antibody C50 (131I C50) on tumor growth and the therapeutic efficacy of combination of low dosage 131I C50 with chemotherapy using 5 fluorouracil (5 FU) on human colorectal cancer xenografts in nude mice.〓METHODS: Human colorectal cancer xenografts with positive CEA expression were established in nude mice with LoVo cell line. 5 FU, 2 775 kBq 131I C50, and 5 FU combined with 131I C50 were given to nude mice through tail vein to treat xenografts at 9th day after implantation of tumor cells. Two of the mice of each group were sacrificed randomly at 7th day after the treatment; and cellular ultrastructure of tumor tissues was examined under electron microscope. Pathological changes of tumor tissues were examined under light microscope. Tumor volume, tumor doubling time, and inhibition rate of each group were calculated. Tumor volumes of all groups at 30th day after implantation were compared with each other. RESULTS: There was significant difference of tumor volumes at 30th day after implantation between each other among control group, chemotherapy group,radioimmunotherapy(RAIT) group,and RAIT＋chemotherapy group (P< 0 001). Tumor doubling time of these groups was prolonged and tumor inhibition rates increased successively. CONCLUSION: Low dosage 131I C50 can inhibit tumor growth of human colorectal cancer xenografts in nude mice. Efficacy of tumor growth inhibition can be enhanced by combination of low dosage 131I C50 with chemotherapy.

关 键 词：结肠直肠肿瘤 放射免疫疗法 辅助化学疗法 

分 类 号：R735.3[医药卫生—肿瘤]