N-琥珀酰亚胺3-[^(125)I]碘苯甲酸酯(S^(125)IB)的放射化学合成  

Synthesis of radioiodinated N-Succinimidyl 3-[^(125)I]Iodobenzoate

作　　者：李俊玲[1] 王丽华[1] 张岚[1] 田海滨[1] 汪勇先[1] 

机构地区：[1]中国科学院上海原子核所放药中心,上海201800

出　　处：《核技术》2003年第3期239-241,共3页Nuclear Techniques

摘　　要：利用Iodogen法成功地对N-琥珀酰亚胺 3-(三正丁基锡)苯甲酸酯(ATE)进行了放射性碘-125标记,利用Sep-Pak硅胶柱实现了干扰蛋白标记的ATE及Iodogen与S125IB的分离,得到了放射性碘间标蛋白质有用的中间体S125IB,标记率93%以上。并研究了影响标记的因素,得出较佳标记条件为:ATE与 Na125I摩尔比6:1、氧化剂 Iodogen用量7mg、室温反应5min。N-Succinimidyl 3-(tri-n-butylstannyl)benzoate (ATE) was radioiodinated using Iodogen as oxidant and useful conjugate S125IB of labeling proteins was obtained . ATE and Iodogen affecting labeling proteins were successfully isolated from S125IB by Sep-Pak silica . The labeling efficiency was more than 93%. Several factors affecting labeling such as labeling time, the amount of Iodogen and the mole ratio of ATE to Na125I, were studied. The better labeling conditions were obtained as follows: mole ratio of ATE to Na125I = 6:1, Iodogen=7mg , labeling time = 5min in room temperature.

关 键 词：IODOGEN法 N-琥珀酰亚胺3-(三正丁基锡)苯甲酸酯 碘125 放射性核素 标记 蛋白质 放射化学合成 N-琥珀酰亚胺3-[^125I]碘苯甲酸酯 

分 类 号：R817.9[医药卫生—影像医学与核医学]