胞外环境Ca^(2+)的变化对培养的人类晶状体上皮细胞的影响  

Influence of alteration of extracellular calcium ions concentration for human lens epithelial cells in culture

作　　者：付玲玲[1] 刘谊[1] 张军军[1] 李胜富[2] 张立[2] 代艳[1] 

机构地区：[1]四川大学华西医学中心眼科,四川成都610041 [2]四川大学华西医学中心病理学实验室,四川成都610041

出　　处：《眼科新进展》2003年第2期100-102,共3页Recent Advances in Ophthalmology

基　　金：中华眼科麦格白内障研究基金资助 (编号 :98-基 -0 1)~~

摘　　要：目的　了解晶状体上皮细胞外 Ca2 +的变化对晶状体上皮细胞的影响。方法　用Bo Bo TM - 3分子探针来标记体外培养的胎儿晶状体上皮细胞的细胞核。用激光扫描共聚焦显微镜观察。结果　随着细胞外 Ca2 +浓度的增加 ,细胞凋亡的数量显著增加。可见凋亡的细胞在形态上出现变圆、染色质浓缩边集、核碎裂、凋亡小体形成等变化。结论　 Ca2 +作为第二信使物质 ,是细胞凋亡信息传递中的关键环节。在此研究中我们发现增加晶状体上皮细胞外 Ca2 + 的浓度可以导致晶状体上皮细胞的凋亡。ObjectiveTo observe the influence of alteration of extracellular calcium ions concentration for human lens epithelial cells in culture.MethodsThe nucleus of fetus lens epithelium in vitro culture was labeled with BoBo TM -3, then observing these cells with laser scanning confocal microscopy. ResultsThe number of apoptosis increased significantly depending on the rise of calcium ions concentration in extracellular environment.The cells of apoptosis becomed round and chromatin collect in the edge of nucleus, then fragmentation of nucleus and formation of apoptotic body .ConclusionActing as a second messenger , Ca 2+ is extremely important in information convection of apoptosis.We have found that increasing extracellular calcium ions concentration can lead to apoptosis of lens epithelial cells in culture.

关 键 词：晶状体上皮细胞 细胞外Ca^2+ 细胞凋亡 

分 类 号：R776[医药卫生—眼科] R361.3[医药卫生—临床医学]