p38MAPK抑制剂SB203580抑制人绒癌JAR细胞的体外侵袭作用  

p38 MAPK inhibitor SB203580 in inhibition of in vitro invision of human choriocarcinoma JAR cells

作　　者：张曦倩[1] 庞战军[1] 陈士岭[1] 邢福祺[1] 

机构地区：[1]第一军医大学南方医院妇产科,广东广州510515

出　　处：《实用肿瘤杂志》2003年第2期95-97,共3页Journal of Practical Oncology

基　　金：973国家重点基础研究发展规划资助项目 ( G19990 5 5 90 3)

摘　　要：目的　研究 p38MAPK通路在人绒癌 JAR细胞体外侵袭中的作用。方法　用细胞 EL ISA法测定 JAR细胞中 p38MAPK的活性变化 ;用 Transwell细胞侵入系统检测细胞的侵袭作用。结果　 PMA呈浓度依赖性地激活 JAR细胞中 p38MAPK。 PMA能促进人绒癌 JAR细胞的体外侵袭作用 ,而 p38特异性抑制剂 SB2 0 35 80抑制了JAR细胞的侵袭能力。结论　 p38MAPK通路在人滋养细胞的侵袭行为以及人绒癌的形成中具有重要作用。p38抑制剂可能会为人绒癌的防治提供新的途径。Objective To investigate the effect of signal passway of p38 mitogen-activated protein kinase(MAPK) for invasion of human choriocarcinoma JAR cells induced by PMA. Methods Protein kinase assay was used to detect the kinase activity stimulated by PMA; Transwell cell invasion system was used to observe the invasion of JAR cells. Results In JAR cells, p38 MAPK was activated by PMA in a dose-dependent manner. Inhibition of p38 MAPK by SB203580, a inhibitor of p38 MAPK blocked PMA-induced in vitro invasion of JAR cells. Conclusion Activation of p38 may contribute to a more invasive phenotype of JAR cells. Targeting of p38 with SB 203580 may provide a novel means of controlling invasion of choriocarcinoma.

关 键 词：绒毛膜癌 肿瘤细胞培养 药物作用 肿瘤浸润 蛋白激酶类 代谢 拮抗剂 抑制剂 

分 类 号：R737.33[医药卫生—肿瘤]