大肠癌组织endostatin的表达与血管生成的关系  

Expression of endostatin in colorectal carcinomas and its relation to angiogenesis

作　　者：孝作祥[1] 郑树[1] 彭佳萍[1] 潘月龙[1] 

机构地区：[1]浙江大学肿瘤研究所,浙江杭州310009

出　　处：《实用肿瘤杂志》2003年第2期109-111,共3页Journal of Practical Oncology

摘　　要：目的　探讨内皮抑制素 ( endostatin)蛋白在大肠癌组织中的表达及其与 VEGF、微血管密度 ( MVD)的关系。方法　应用免疫组化 S- P法 ,检测 6 8例大肠癌及其正常大肠组织 endostatin表达、VEGF表达及 MVD。结果　 endostatin蛋白可在大肠癌组织的肿瘤细胞、内皮细胞及浸润淋巴细胞中表达。 endostatin表达与 Dukes分期密切相关。endostatin高表达组的 MVD( 12 .35± 4.96 )明显低于 endostatm低表达组 ( 16 .38± 6 .2 8)。 VEGF染色阳性组与阴性组的 endostatin蛋白表达差异无显著性。结论　 endostatin在大肠癌组织的不同类型细胞中出现 ,与大肠癌血管生成密切相关 ,endostatin的表达水平随着 Dukes分期的进展而降低反映了血管生成促进因子和抑制因子之间平衡的转变。Objective To investigate the expression of endostatin in colorectal carcinomas and its relation to vascular endothelial growth factor (VEGF) and microvascular density (MVD). Methods The expressions of endostatin, VEGF and MVD were detected in 68 cases of colorectal carcinomas by immunohistochemistry. Results Endostatin immunoreactivity was detected in tumor cells, endothelial cells, lymphocytes of colorectal carcinomas. The Dukes stage was significantly associated with endostatin expression; MVD level was lower in the higher endostatin protein expression group (12.35±4.96) than that of the lower endostatin protein expression group (16.38±6.28). Conclusion Endostatin is present in various cell types in colorectal carcinoma and is closely related to angiogenesis.;Key words:colorectal neoplasms/pathology; neovascularization,pathologic; endothelial growth factors/antagonists and inhibitors; angiogenesis factors/biosynthesis; gene expression; gene therapy5BZ]YAO Hai-Jun, LIU Xian-Ming, ZHANG Xiao-Gang*, XIA Xi (Institute of Applied Chemistry,Xinjiang University,Urumqi 830046) Abstract Nanosized PbTiO 3 powder was prepared by a sol-gel method and characterized by XRD, TEM and CV techniques. PbTiO 3 modified MnO 2 electrodes were fabricated and their electrochemical behavior were studied by means of galvonostatic charge-discharge and CV experiments. The results indicated that the nano- sized PbTiO 3 forms a series of complex oxides with MnO 2 of different valency and prevents the formation of Mn 3O 4 during the course of electrode reaction. The discharge capacity of MnO 2 electrodes modified with 5 0%(mass fraction) nanophase PbTiO 3 is in average 45% higher than that of I.C.No.1 in 40%(mass fraction) KOH electrolytes, and charge- discharge efficiency is much improved by 10%～40%.

关 键 词：结肠直肠肿瘤 病理学 新生血管化 病理性 内皮生成因子 拮抗剂 抑制剂 血管生成因子 生物合成 基因表达 基因疗法 

分 类 号：R735.34[医药卫生—肿瘤]