血管性痴呆大鼠海马结构突触膜糖蛋白的变化及药物干预  

Changes of Hippocampal Synaptophysin in Rats of Vascular Dementia and Drug Intervention

作　　者：赵小贞[1] 王玮[1] 康仲涵[1] 陈金水[2] 徐剑文[1] 陈春鹏[1] 吴天敏[2] 

机构地区：[1]福建医科大学人体解剖学与组织胚胎学系,福州350004 [2]福建医科大学附属第一医院中医科,福州350005

出　　处：《福建医科大学学报》2003年第1期44-47,T004,共5页Journal of Fujian Medical University

基　　金：福建省教育厅科研基金资助项目 ( JA0 0 196)

摘　　要：目的　观察血管性痴呆雌性大鼠海马结构突触膜糖蛋白的变化及中药黄精口服液对其的影响。　方法　以结扎 SD雌性大鼠双侧颈总动脉方法建立血管性痴呆模型 ,采用 Morris水迷宫检测大鼠学习记忆能力 ,黄精口服液 (0 .5 m L / 10 0 g)灌胃 ,应用免疫组织化学法和图像分析技术测定海马结构突触膜糖蛋白免疫反应产物灰度值。　结果　痴呆组和痴呆 +生理盐水组海马结构突触膜糖蛋白免疫反应产物灰度值比空白对照组明显降低 ;痴呆 +黄精组高于痴呆组和痴呆 +生理盐水组 (P<0 .0 5 ) ;大鼠平均逃避潜伏期与海马结构突触膜糖蛋白呈负相关。　结论　海马结构突触膜糖蛋白减少是血管性痴呆神经病理机制之一 ;黄精口服液具有重塑突触、改善Objective\ To observe the change of hippocampal synaptophysin in female rats of vascular dementia and to study the effect of polygonatum oral liquid on vascular dementia. \ Methods\ The animal model was reproduced by permanent occlusion of bilateral common carotid arteries of rats.\ Learning and memory of rats were tested by Morris water maze.\ Immunohistochemistry technique and images analysis were used to study the synaptophysin express in hippocampal formation.\ Results\ The synaptophysin immunolabeling density of hippocampal decreased in vascular dementia rats in comparison with normal rats.\ The expression of synaptophysin in rats that was treated by polygonatum oral liquid was significantly higher than those in both vascular dementia rats and rats treated by saline.\ Moreover, the escape latency of rats is correlated with the synaptophysin immunolabeling density of hippocampal formation.\ Conclusion\ Polygonatum oral liquid display the function of improving synaptic remodelling and promotes learning and memory in rats with vascular dementia.\;

关 键 词：血管性痴呆 突触膜糖蛋白 黄精 海马 大鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]